Label-free microscopy of mitotic chromosomes using the polarization orthogonality breaking technique
Résumé
We report how a recently developed polarization imaging technique, implementing micro-wave photonics and referred to as orthogonality-breaking (OB) imaging, can be adapted on a classical confocal fluorescence microscope, and is able to provide informative polarization images from a single scan of the cell sample. For instance, the comparison of the images of various cell lines at different cell-cycle stages obtained by OB polarization microscopy and fluorescence confocal images shows that an endogenous polarimetric contrast arizes with this instrument on compacted chromosomes during cell division.
Mots clés
Cell culture
Cell proliferation
Fluorescence
Imaging techniques
Polarization
Cell divisions
Confocal fluorescence microscope
Confocal image
Mitotic chromosomes
Orthogonality
Polarization images
Polarization imaging
Polarization microscopy
Chromosomes
hoe 33342
paraformaldehyde
penicillin derivative
phosphate buffered saline
streptomycin
anisotropy
Article
cell division
chromosome
confocal laser scanning microscopy
controlled study
fluorescence imaging
histology
human
human cell
immunoblotting
live cell imaging
mitosis
osteosarcoma
polarization microscopy
signal noise ratio
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Desapogu-2021-Label-free microscopy of mitotic chromosomes.pdf (8.07 Mo)
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| Commentaire | Data underlying the results presented in this paper are not publicly available at this time but may be obtained from the authors upon reasonable request. |
