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Article Dans Une Revue Scientific Reports Année : 2021

Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol

Maximilien Clabaut
  • Fonction : Auteur
Amine Boukerb
Amine Ben Mlouka
  • Fonction : Auteur
Amandine Suet
  • Fonction : Auteur
Ali Tahrioui
  • Fonction : Auteur
Magalie Barreau
  • Fonction : Auteur
Olivier Maillot
  • Fonction : Auteur
Agathe Le Tirant
  • Fonction : Auteur
Madina Karsybayeva
  • Fonction : Auteur
Coralie Kremser
  • Fonction : Auteur
Gérard Redziniak
  • Fonction : Auteur
Cécile Duclairoir-Poc
Chantal Pichon
  • Fonction : Auteur
Julie Hardouin
Pascal Cosette
Sylvie Chevalier
  • Fonction : Auteur
Marc Feuilloley

Résumé

Abstract We previously showed that the physiological concentration of 17β-estradiol in the vaginal environment is sufficient to affect the membrane dynamics and adhesion phenotype of the Lactobacillus crispatus strain CIP104459. However, L. crispatu s is a heterogeneous species. Here, we investigated the effect of 17β-estradiol on the recently isolated L. crispatus vaginal strain V4, related to a cluster distant from CIP104459 and at the limit of being a different subspecies. Grown in the same medium, the two strains expressed a highly similar pool of proteins. However, in contrast to CIP104459, L. crispatus V4 showed high aggregation potential and 17β-estradiol promoted this phenotype. This effect was associated with large changes in cell-surface polarity and Lewis acid/base properties. In addition, we observed no effect on the membrane dynamics, contrary to CIP104459. These results can be explained by differences in the properties and organization of the S layer between the two strains. However, as for CIP104459, 17β-estradiol increased biosurfactant production of L. crispatus V4 and their adhesion to vaginal cells. This suggests that 17β-estradiol agonists would be valuable tools to favor a stable re-implantation of L. crispatus in the vaginal mucosa.

Dates et versions

hal-03326611 , version 1 (26-08-2021)

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Citer

Maximilien Clabaut, Amine Boukerb, Amine Ben Mlouka, Amandine Suet, Ali Tahrioui, et al.. Variability of the response of human vaginal Lactobacillus crispatus to 17β-estradiol. Scientific Reports, 2021, 11 (1), ⟨10.1038/s41598-021-91017-5⟩. ⟨hal-03326611⟩
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