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Article Dans Une Revue Nucleic Acids Research Année : 2021

Translational accuracy of a tethered ribosome

Résumé

Ribosomes are evolutionary conserved ribonucleoprotein complexes that function as two separate subunits in all kingdoms. During translation initiation, the two subunits assemble to form the mature ribosome, which is responsible for translating the messenger RNA. When the ribosome reaches a stop codon, release factors promote translation termination and peptide release, and recycling factors then dissociate the two subunits, ready for use in a new round of translation. A tethered ribosome, called Ribo-T, in which the two subunits are covalently linked to form a single entity, was recently described in Escherichia coli. A hybrid ribosomal RNA (rRNA) consisting of both the small and large subunit rRNA sequences was engineered. The ribosome with inseparable subunits generated in this way was shown to be functional and to sustain cell growth. Here, we investigated the translational properties of Ribo-T. We analyzed its behavior during amino acid misincorporation, −1 or +1 frameshifting, stop codon readthrough, and internal translation initiation. Our data indicate that covalent attachment of the two subunits modifies the properties of the ribosome, altering its ability to initiate and terminate translation correctly.
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Dates et versions

hal-03323523 , version 1 (21-08-2021)

Identifiants

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Celine Fabret, Olivier Namy. Translational accuracy of a tethered ribosome. Nucleic Acids Research, 2021, 49, pp.5308 - 5318. ⟨10.1093/nar/gkab259⟩. ⟨hal-03323523⟩
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