Subcellular imaging during single cell mechanical characterization - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2020

Subcellular imaging during single cell mechanical characterization

Résumé

We report a method for combining confocal microscopy with single-cell mechanical characterization. This method allows investigating the effect of subcellular deformation (e.g. membranes, cytoplasm, cytoskeleton and organelles as endoplasmic reticulum, mitochondria, and nucleus) on cell mechanical properties (e.g. stiffness and viscosity). Such a method is essential to choose biologically relevant mechanical properties of malignant cells as diagnostic cancer biomarkers. Using MEMS tweezers, we captured live single cancer cells and performed subcellular imaging during compression assays for mechanical measurements to obtain the deformation-dependent cell properties.
Fichier non déposé

Dates et versions

hal-03322819 , version 1 (19-08-2021)

Identifiants

Citer

Deniz Pekin, Gregoire Perret, Quentin Rezard, Jean Claude Gerbedoen, Samuel Meignan, et al.. Subcellular imaging during single cell mechanical characterization. 33rd IEEE International Conference on Micro Electro Mechanical Systems (MEMS), Jan 2020, Vancouver, Canada. pp.62-65, ⟨10.1109/MEMS46641.2020.9056362⟩. ⟨hal-03322819⟩
25 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More