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Communication Dans Un Congrès Année : 2021

Feed restriction affects protein and microRNA levels in dairy cows' milk

M. Gelé
  • Fonction : Auteur

Résumé

The aim of this study was to observe the effect of feed restriction (FR) on milk concentrations of major proteins and microRNA in order to identify potential biomarkers of negative energy balance in cows. We hypothesized that the effects of FR on mammary gland metabolism are reflected in milk and its different fractions. Two FR trials were conducted at mid lactation to induce negative energy balance: a short duration and high intensity one (SH) and a long duration and moderate intensity one (LM). The SH experiment involved 8 Holstein cows. The LM experiment involved 19 Holstein cows either in control (n=9) or restriction conditions (n=10). FR lasted 6 and 29 days with a dry matter intake reduced by 64 and 20% of ad libitum intake, and induced a negative energy balance of-42 and-21 MJ/d, respectively for SH and LM experiments. Milk was sampled before, during (3 times for LM) and after the restriction periods. MicroRNA, extracted from whole milk, fat globules, milk isolated mammary epithelial cells and extracellular vesicles were sequenced and protein concentrations analyzed using LC-MS. Concentration of several major proteins decreased during FR: αS2-casein (LM and SH), αS1-casein (SH), κ-casein (LM), α-lactalbumin (LM), β-lactoglobulin (LM). Among 2896 microRNA detected in milk, the abundance of 159 microRNA was affected by FR, mostly in extracellular vesicles (n=83) and whole milk (n=85), few in fat globules (n=6), but none in mammary epithelial cells. Among these 159 microRNA, 15 varied in two compartments. These experiments showed that FR affects the abundance of proteins and microRNA in milk. It may reflect modifications of mammary gland metabolism. These results will be further explored by proteomic analysis and by RT-qPCR on more samples. These preliminary results are promising in the search for non-invasive biomarkers of negative energy balance in milk.
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Dates et versions

hal-03318595 , version 1 (10-08-2021)

Identifiants

  • HAL Id : hal-03318595 , version 1

Citer

Antoine Leduc, Sandrine Le Guillou, L Bianchi, M. Gelé, Lucile Herve, et al.. Feed restriction affects protein and microRNA levels in dairy cows' milk. 72. Annual meeting of the european federation of animal science (EAAP), Aug 2021, Davos, Switzerland. pp.620. ⟨hal-03318595⟩
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