High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project: one size fits all? - Archive ouverte HAL
Article Dans Une Revue Acta crystallographica Section D : Structural biology [1993-...] Année : 2005

High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project: one size fits all?

Nicolas Leulliot
  • Fonction : Auteur
  • PersonId : 1106361
Lionel Trésaugues
  • Fonction : Auteur
Michael Bremang
  • Fonction : Auteur
Isabelle Sorel
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Nathalie Ulryck
  • Fonction : Auteur
Ilham Aboulfath
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Anne Poupon
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Dominique Liger
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Sophie Quevillon-Cheruel
Joël Janin
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Herman van Tilbeurgh
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  • PersonId : 1106362

Résumé

Crystallization has long been regarded as one of the major bottlenecks in highthroughput structural determination by X-ray crystallography. Structural genomics projects have addressed this issue by using robots to set up automated crystal screens using nanodrop technology. This has moved the bottleneck from obtaining the first crystal hit to obtaining diffraction-quality crystals, as crystal optimization is a notoriously slow process that is difficult to automatize. This article describes the high-throughput optimization strategies used in the Yeast Structural Genomics project, with selected successful examples.

Dates et versions

hal-03299325 , version 1 (27-07-2021)

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Citer

Nicolas Leulliot, Lionel Trésaugues, Michael Bremang, Isabelle Sorel, Nathalie Ulryck, et al.. High-throughput crystal-optimization strategies in the South Paris Yeast Structural Genomics Project: one size fits all?. Acta crystallographica Section D : Structural biology [1993-..], 2005, 61, pp.664 - 670. ⟨10.1107/s0907444905000028⟩. ⟨hal-03299325⟩
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