Lanthanide-Containing Polymer Nanoparticles for Biological Tagging Applications: Nonspecific Endocytosis and Cell Adhesion
Résumé
We describe the synthesis and characterization of element-encoded polystyrene nanoparticles
with diameters on the order of 100 nm and a narrow size distribution. Individual particles contain ca. 103
chelated lanthanide ions, of either a single element or a mixture of elements. These particles were effectively
internalized by nonspecific endocytosis into three cell lines associated with human leukemia. Using an
assay based upon ICP-MS detection, we could monitor quantitatively cell adhesion induced by cell
differentiation of THP-1 cells in response to phorbol ester stimulation (PMA) in single cell type or mixed
cultures.