H-NS Silencing of the Salmonella Pathogenicity Island 6-Encoded Type VI Secretion System Limits Salmonella enterica Serovar Typhimurium Interbacterial Killing - Archive ouverte HAL Access content directly
Journal Articles Infection and Immunity Year : 2015

H-NS Silencing of the Salmonella Pathogenicity Island 6-Encoded Type VI Secretion System Limits Salmonella enterica Serovar Typhimurium Interbacterial Killing

Yannick Brunet
  • Function : Author
Ahmad Khodr
  • Function : Author
Laureen Logger
  • Function : Author
Laurent Aussel
  • Function : Author
Tâm Mignot
Sylvie Rimsky
  • Function : Author

Abstract

ABSTRACT The secretion of bacterial toxin proteins is achieved by dedicated machineries called secretion systems. The type VI secretion system (T6SS) is a widespread versatile machine used for the delivery of protein toxins to both prokaryotic and eukaryotic cells. In Salmonella enterica serovar Typhimurium, the expression of the T6SS genes is activated during macrophage or mouse infection. Here, we show that the T6SS gene cluster is silenced by the histone-like nucleoid structuring H-NS protein using a combination of reporter fusions, electrophoretic mobility shift assays, DNase footprinting, and fluorescence microscopy. We further demonstrate that derepression of the S . Typhimurium T6SS genes induces T6SS-dependent intoxication of competing bacteria. Our results suggest that relieving T6SS H-NS silencing may be used as a sense-and-kill mechanism that will help S . Typhimurium to homogenize and synchronize the microbial population to gain efficiency during infection.

Dates and versions

hal-03274770 , version 1 (30-06-2021)

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Yannick Brunet, Ahmad Khodr, Laureen Logger, Laurent Aussel, Tâm Mignot, et al.. H-NS Silencing of the Salmonella Pathogenicity Island 6-Encoded Type VI Secretion System Limits Salmonella enterica Serovar Typhimurium Interbacterial Killing. Infection and Immunity, 2015, 83 (7), pp.2738-2750. ⟨10.1128/IAI.00198-15⟩. ⟨hal-03274770⟩
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