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Article Dans Une Revue Phytopathogenic Mollicutes Année : 2019

Isolating intact phytoplasma particles from plants for sequencing applications and for testing host responses to phytoplasmas

Vered Naor
  • Fonction : Auteur
Ofir Bahar
  • Fonction : Auteur
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Résumé

Candidatus Phytoplasma species are obligate plant parasites limited to the phloem tissue. The difficulties to grow phytoplasma in culture seriously impedes the study of these prokaryotes. In this study, a protocol to extract intact phytoplasmas from infected periwinkle plants using filtration, differential and density-gradient centrifugations was optimized. Confocal and transmission electron microscopy was used to visualize intact phytoplasmas in the preparation. Quantitative-PCR and DNA-seq analyses revealed that phytoplasma DNA was significantly enriched following the extraction procedure. Challenging periwinkle plants with phytoplasma preparation induced the expression of one defence-related gene. This study provides an optimized procedure for phytoplasma DNA enrichment and for extracting intact phytoplasmas that can be used to examine host responses to them.
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Dates et versions

hal-03270218 , version 1 (31-08-2021)

Identifiants

Citer

Nofar Assoline, Diego Santos-Garcia, Vered Naor, Ofir Bahar. Isolating intact phytoplasma particles from plants for sequencing applications and for testing host responses to phytoplasmas. Phytopathogenic Mollicutes, 2019, 9 (1), pp.125-126. ⟨10.5958/2249-4677.2019.00063.X⟩. ⟨hal-03270218⟩
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