Colicin A Immunity Protein Interacts with the Hydrophobic Helical Hairpin of the Colicin A Channel Domain in the Escherichia coli Inner Membrane - Archive ouverte HAL Access content directly
Journal Articles Journal of Bacteriology Year : 2001

Colicin A Immunity Protein Interacts with the Hydrophobic Helical Hairpin of the Colicin A Channel Domain in the Escherichia coli Inner Membrane

Angèle Nardi
  • Function : Author
Yves Corda
Daniel Baty
  • Function : Author

Abstract

ABSTRACT The colicin A pore-forming domain (pfColA) was fused to a bacterial signal peptide (sp-pfColA). This was inserted into the Escherichia coli inner membrane in functional form and could be coimmunoprecipitated with epitope-tagged immunity protein (EpCai). We constructed a series of fusion proteins in which various numbers of sp-pfColA α-helices were fused to alkaline phosphatase (AP). We showed that a fusion protein made up of the hydrophobic α-helices 8 and 9 of sp-pfColA fused to AP was specifically coimmunoprecipitated with EpCai produced in the same cells. This is the first biochemical evidence that Cai recognizes and interacts with the colicin A hydrophobic helical hairpin.

Dates and versions

hal-03269524 , version 1 (24-06-2021)

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Angèle Nardi, Yves Corda, Daniel Baty, Denis Duché. Colicin A Immunity Protein Interacts with the Hydrophobic Helical Hairpin of the Colicin A Channel Domain in the Escherichia coli Inner Membrane. Journal of Bacteriology, 2001, 183 (22), pp.6721-6725. ⟨10.1128/JB.183.22.6721-6725.2001⟩. ⟨hal-03269524⟩
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