Monitoring the purification by high-performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase-sensitive two-dimensional nuclear magnetic resonance - Archive ouverte HAL Access content directly
Journal Articles European Journal of Biochemistry Year : 1987

Monitoring the purification by high-performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase-sensitive two-dimensional nuclear magnetic resonance

Abstract

High-resolution phase-sensitive two-dimensional proton nuclear magnetic resonance was used to monitor the preparation by high-performance liquid chromatography of homogeneous proteins from the venom of Naja mossambica mossambica. This resulted in the characterization of a heterogeneous protein preparation VII2, which had been used in earlier structural studies by NMR, as well as a homogeneous protein CTXIIb and a nearly homogeneous protein fraction CTXIIa, which are now both subject to further investigations of their solution conformations.

Dates and versions

hal-03261768 , version 1 (16-06-2021)

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Gottfried Otting, P. Marchot, Pierre E Bougis, Hervé Rochat, Kurt Wüthrich. Monitoring the purification by high-performance liquid chromatography of cardiotoxins from Naja mossambica mossambica using phase-sensitive two-dimensional nuclear magnetic resonance. European Journal of Biochemistry, 1987, 168 (3), pp.603-607. ⟨10.1111/j.1432-1033.1987.tb13459.x⟩. ⟨hal-03261768⟩

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