Automated ChIPmentation procedure on limited biological material of the human blood fluke Schistosoma mansoni
Résumé
Automated ChIPmentation procedure is a convenient alternative to native chromatin immunoprecipitation (N-ChIP). It is now routinely used for ChIP-Seq. Using the human parasite Schistosoma mansoni , whose production requires scarifying animals and should therefore kept to a minimum, we show here that the automated ChIPmentation is suitable for limited biological material. We define as operational limit ≥20,000 cells. We also present a streamlined protocol for the preparation of ChIP input libraries.
Domaines
Parasitologie| Origine | Fichiers produits par l'(les) auteur(s) |
|---|