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Journal Articles Thrombosis and Haemostasis Year : 2012

Functional mapping of the A2 domain from human factor VIII

Abstract

Summary Coagulation factor VIII (FVIII) is a multidomain glycoprotein in which the FVIII A2 domain is a key structural element. We aimed at identifying residues within FVIII A2 domain that are crucial for the maintenance of the cofactor function. A high number (n=206) of mutants were generated by substituting original residues with alanine. The mutants were expressed in COS-1 cells and their antigen levels and procoagulant activities were measured. The residues were classified in three categories: those with a non-detrimental alteration of their activities (activity >50 % of control FVIII; n=98), those with a moderate alteration (15 %
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hal-03247627 , version 1 (03-06-2021)

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Jean-Luc Plantier, Didier Saboulard, Jean-Luc Pellequer, Claude Négrier, Marc Delcourt. Functional mapping of the A2 domain from human factor VIII. Thrombosis and Haemostasis, 2012, 107 (02), pp.315-327. ⟨10.1160/TH11-07-0492⟩. ⟨hal-03247627⟩
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