Tillandsia is a genus belonging to the Bromeliaceae family, most of which are epiphytes. The flowers of some of theTillandsia species are very fragrant, but the volatile composition has been scarcely reported. In this report, we studied the chemical composition of volatile compounds emitted by the flowers of Tillandsia xiphioides using the HS-SPME/GC-MS method. The extraction conditions (fiber, temperature, and time) were optimized using a multivariate approach, and the composition of the extracted volatiles was determined by gas chromatography coupled with mass spectrometry (GC-MS). In total, 30 extracted compounds were identified. Two extraction methods are necessary for the efficient extraction of the volatile compounds. These results were applied to profile two forms of T. xiphioides