Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs - Archive ouverte HAL Access content directly
Journal Articles Nature Communications Year : 2021

Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs

Bastien Bissaro
Thu Vuong
  • Function : Author
Jean-Guy Berrin

Abstract

Abstract Oxidative plant cell-wall processing enzymes are of great importance in biology and biotechnology. Yet, our insight into the functional interplay amongst such oxidative enzymes remains limited. Here, a phylogenetic analysis of the auxiliary activity 7 family (AA7), currently harbouring oligosaccharide flavo-oxidases, reveals a striking abundance of AA7-genes in phytopathogenic fungi and Oomycetes. Expression of five fungal enzymes, including three from unexplored clades, expands the AA7-substrate range and unveils a cellooligosaccharide dehydrogenase activity, previously unknown within AA7. Sequence and structural analyses identify unique signatures distinguishing the strict dehydrogenase clade from canonical AA7 oxidases. The discovered dehydrogenase directly is able to transfer electrons to an AA9 lytic polysaccharide monooxygenase (LPMO) and fuel cellulose degradation by LPMOs without exogenous reductants. The expansion of redox-profiles and substrate range highlights the functional diversity within AA7 and sets the stage for harnessing AA7 dehydrogenases to fine-tune LPMO activity in biotechnological conversion of plant feedstocks.
Fichier principal
Vignette du fichier
s41467-021-22372-0.pdf (2.09 Mo) Télécharger le fichier
Origin : Publisher files allowed on an open archive

Dates and versions

hal-03234953 , version 1 (26-05-2021)

Licence

Attribution

Identifiers

Cite

Majid Haddad Momeni, Folmer Fredslund, Bastien Bissaro, Olanrewaju Raji, Thu Vuong, et al.. Discovery of fungal oligosaccharide-oxidising flavo-enzymes with previously unknown substrates, redox-activity profiles and interplay with LPMOs. Nature Communications, 2021, 12 (1), ⟨10.1038/s41467-021-22372-0⟩. ⟨hal-03234953⟩
131 View
58 Download

Altmetric

Share

Gmail Facebook X LinkedIn More