Elucidating venlafaxine metabolism in the Mediterranean mussel (Mytilus galloprovincialis) through combined targeted and non-targeted approaches - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Science of the Total Environment Année : 2021

Elucidating venlafaxine metabolism in the Mediterranean mussel (Mytilus galloprovincialis) through combined targeted and non-targeted approaches

Frédérique Courant
Thibaut Dumas
H. Fenet
  • Fonction : Auteur
  • PersonId : 1060749
  • IdRef : 113040601
Elena Gomez

Résumé

Exposure of aquatic organisms to antidepressants is currently well documented, while little information is available on how wild organisms cope with exposure to these pharmaceutical products. Studies on antidepressant metabolism in exposed organisms could generate information on their detoxification pathways and pharmacokinetics. The goal of this study was to enhance knowledge on the metabolism of venlafaxine (VEN)-an antidepressant frequently found in aquatic ecosystems-in Mytilus galloprovincialis, a bivalve that is present worldwide. An original tissue extraction technique based on the cationic properties of VEN was developed for further analysis of VEN and its metabolites using targeted and non-targeted approaches. This extraction method was assessed in terms of recovery and matrix effects for VEN metabolites. Commercial analytical standards were applied to characterize metabolites found in mussels exposed to 10 μg/L VEN for 3 and 7 days. Targeted and non-targeted approaches using liquid chromatography (LC) combined with high-resolution mass spectrometry (HRMS) were implemented to screen for expected metabolites based on the literature on aquatic species, and for metabolites not previously documented. Four venlafaxine metabolites were identified, namely N-desmethylvenlafaxine and O-desmethylvenlafaxine, which were clearly identified using analytical standards, and two other metabolites revealed by non-target analysis. According to the signal intensity, hydroxy-venlafaxine (OH-VEN) was the predominant metabolite detected in mussels exposed for 3 and 7 days.
Fichier principal
Vignette du fichier
S0048969721014558.pdf (481.16 Ko) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03228661 , version 1 (24-04-2023)

Licence

Identifiants

Citer

N. Ariza-Castro, Frédérique Courant, Thibaut Dumas, Bénédicte Marion, H. Fenet, et al.. Elucidating venlafaxine metabolism in the Mediterranean mussel (Mytilus galloprovincialis) through combined targeted and non-targeted approaches. Science of the Total Environment, 2021, 779, pp.146387. ⟨10.1016/j.scitotenv.2021.146387⟩. ⟨hal-03228661⟩
25 Consultations
51 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More