Phenotypic landscape of granulocytes and monocytes by multiparametric flow cytometry: A prospective study of a 1-tube panel strategy for diagnosis and prognosis of patients with MDS - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cytometry Part B: Clinical Cytometry Année : 2020

Phenotypic landscape of granulocytes and monocytes by multiparametric flow cytometry: A prospective study of a 1-tube panel strategy for diagnosis and prognosis of patients with MDS

Sylvain Barreau
  • Fonction : Auteur
Alexa S. Green
  • Fonction : Auteur
Charles Dussiau
Anna Raimbault
Lise Willems
  • Fonction : Auteur
Didier Bouscary
  • Fonction : Auteur
Olivier Kosmider
Nicolas Chapuis
  • Fonction : Auteur correspondant
  • PersonId : 1037061

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Résumé

Background: Multiparametric flow cytometry (MFC) was recently reported to be a helpful additional tool in the diagnosis of myelodysplastic syndromes (MDS). However, numerous aberrancies have been reported that makes their evaluation difficult as part of a routine diagnosis. Methods: Here, we validated a 1-tube panel for the evaluation of granulocytic and monocytic maturation by MFC and correlated our findings with diagnosis and prognosis of MDS. A total of 251 samples with MDS suspicion were prospectively analyzed and compared to an internal reference database leading to the calculation of the Diff score. Results: The associated specificity and sensitivity values of this scoring system were 92.1% and 60.4% in a first learning cohort and 96.7% and 65.2% in a second independent validation cohort. The combination of the Diff score with the concomitantly calculated Ogata score increased the sensitivity to 74.2% and 78.3% in the learning and validation cohorts, respectively. Finally, a normal Diff score in MDS patients was associated with a significant prolonged progression-free survival. Conclusions: Taken together, the present data indicate that our strategy is a sensitive and specific MFC tool for the diagnosis of MDS-related cytopenia(s) which could be also useful for predicting evolution of these diseases.

Dates et versions

hal-03227850 , version 1 (17-05-2021)

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Sylvain Barreau, Alexa S. Green, Charles Dussiau, Anne Sophie Alary, Anna Raimbault, et al.. Phenotypic landscape of granulocytes and monocytes by multiparametric flow cytometry: A prospective study of a 1-tube panel strategy for diagnosis and prognosis of patients with MDS. Cytometry Part B: Clinical Cytometry, 2020, 98 (3), pp.226-237. ⟨10.1002/cyto.b.21843⟩. ⟨hal-03227850⟩
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