<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03223915</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-15T15:36:09+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">A New Approach to Produce Succinic Acid Through a Co-Culture System</title>
            <author role="aut">
              <persName>
                <forename type="first">Alaa</forename>
                <surname>Salma</surname>
              </persName>
              <email type="md5">707c9607058943095f1e5e5f3374598d</email>
              <email type="domain">unilasalle.fr</email>
              <idno type="idhal" notation="string">alaa-salma</idno>
              <idno type="idhal" notation="numeric">1286039</idno>
              <idno type="halauthorid" notation="string">2206321-1286039</idno>
              <affiliation ref="#struct-194938"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Rawa</forename>
                <surname>Abdallah</surname>
              </persName>
              <email type="md5">8ed789aa3e8d640e2528ecd0ca204bec</email>
              <email type="domain">univ-rennes1.fr</email>
              <idno type="idhal" notation="numeric">963653</idno>
              <idno type="halauthorid" notation="string">847146-963653</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-4262-0382</idno>
              <affiliation ref="#struct-303340"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Florence</forename>
                <surname>Fourcade</surname>
              </persName>
              <idno type="halauthorid">699621-0</idno>
              <affiliation ref="#struct-194938"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Abdeltif</forename>
                <surname>Amrane</surname>
              </persName>
              <email type="md5">0d99a442446f0b47e7d42912f06bccde</email>
              <email type="domain">univ-rennes1.fr</email>
              <idno type="idhal" notation="string">abdeltif-amrane</idno>
              <idno type="idhal" notation="numeric">768602</idno>
              <idno type="halauthorid" notation="string">713977-768602</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-2622-2384</idno>
              <idno type="IDREF">https://www.idref.fr/07491412X</idno>
              <affiliation ref="#struct-194938"/>
            </author>
            <author role="crp">
              <persName>
                <forename type="first">Hayet</forename>
                <surname>Djelal</surname>
              </persName>
              <email type="md5">070aca28bbec3cc5fc5de9941afff15d</email>
              <email type="domain">unilasalle.fr</email>
              <idno type="idhal" notation="string">hayet-djelal</idno>
              <idno type="idhal" notation="numeric">1157029</idno>
              <idno type="halauthorid" notation="string">704127-1157029</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-5619-2633</idno>
              <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=CNTgCIgAAAAJ</idno>
              <affiliation ref="#struct-1069231"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Laurent</forename>
                <surname>Jonchère</surname>
              </persName>
              <email type="md5">dc1f1e9bdb3dc2065f8bf1bfd5663118</email>
              <email type="domain">univ-rennes.fr</email>
            </editor>
            <funder>This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.</funder>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2021-06-18 16:20:18</date>
              <date type="whenModified">2025-07-04 09:16:03</date>
              <date type="whenReleased">2021-06-21 16:00:28</date>
              <date type="whenProduced">2021-09</date>
              <date type="whenEndEmbargoed">2021-11-03</date>
              <ref type="file" target="https://hal.science/hal-03223915v1/document">
                <date notBefore="2021-11-03"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://hal.science/hal-03223915v1/file/Salma_et%20al_2021_new%20approach%20to%20produce%20succinic_accepted.pdf" id="file-3264879-2870101">
                <date notBefore="2021-11-03"/>
              </ref>
              <ref type="annex" n="0" target="https://hal.science/hal-03223915v1/file/Salma_et%20al_2021_new%20approach%20to%20produce%20succinic_suppl%20data.pdf" id="file-3264879-2870102">
                <date notBefore="2021-11-03"/>
              </ref>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="113861">
                <persName>
                  <forename>Laurent</forename>
                  <surname>Jonchère</surname>
                </persName>
                <email type="md5">dc1f1e9bdb3dc2065f8bf1bfd5663118</email>
                <email type="domain">univ-rennes.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03223915</idno>
            <idno type="halUri">https://hal.science/hal-03223915</idno>
            <idno type="halBibtex">salma:hal-03223915</idno>
            <idno type="halRefHtml">&lt;i&gt;Applied Biochemistry and Biotechnology&lt;/i&gt;, 2021, 193 (9), pp.2872-2892. &lt;a target="_blank" href="https://dx.doi.org/10.1007/s12010-021-03572-2"&gt;&amp;#x27E8;10.1007/s12010-021-03572-2&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Applied Biochemistry and Biotechnology, 2021, 193 (9), pp.2872-2892. &amp;#x27E8;10.1007/s12010-021-03572-2&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-3264879-2870101"/><ref corresp="#file-3264879-2870102"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="UNIV-RENNES1">Université de Rennes 1</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="INSA-RENNES">Institut National des Sciences Appliquées de Rennes</idno>
            <idno type="stamp" n="ENSC-RENNES">Ecole Nationale Supérieure de Chimie de Rennes</idno>
            <idno type="stamp" n="ISCR">INSTITUT DES SCIENCES CHIMIQUES DE RENNES</idno>
            <idno type="stamp" n="SCR-CIP" corresp="ISCR">Chimie et Ingénierie des Procédés</idno>
            <idno type="stamp" n="STATS-UR1">Statistiques-HAL-UR1</idno>
            <idno type="stamp" n="COMUE-NORMANDIE">Normandie Université</idno>
            <idno type="stamp" n="UR1-SPM">Université de Rennes 1 - UFR Sciences et Propriétés de la Matière</idno>
            <idno type="stamp" n="INC-CNRS">Institut de Chimie du CNRS</idno>
            <idno type="stamp" n="UR1-UFR-SPM">UFR SPM Sciences et Propriétés de la Matière</idno>
            <idno type="stamp" n="UR1-HAL">Publications labos UR1 dans HAL-Rennes 1</idno>
            <idno type="stamp" n="UR1-SDLM">UR1 - publications SDLM</idno>
            <idno type="stamp" n="TEST-UR-CSS">TEST Université de Rennes CSS</idno>
            <idno type="stamp" n="UNIV-RENNES">Université de Rennes</idno>
            <idno type="stamp" n="INSA-GROUPE">Groupe INSA</idno>
            <idno type="stamp" n="UNILASALLE">UniLaSalle</idno>
            <idno type="stamp" n="TEST-HALCNRS">Collection test HAL CNRS</idno>
            <idno type="stamp" n="UR1-MMS">Pôle UnivRennes - Matériaux</idno>
            <idno type="stamp" n="CYCLANN">CYCLANN</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">A New Approach to Produce Succinic Acid Through a Co-Culture System</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Alaa</forename>
                    <surname>Salma</surname>
                  </persName>
                  <email type="md5">707c9607058943095f1e5e5f3374598d</email>
                  <email type="domain">unilasalle.fr</email>
                  <idno type="idhal" notation="string">alaa-salma</idno>
                  <idno type="idhal" notation="numeric">1286039</idno>
                  <idno type="halauthorid" notation="string">2206321-1286039</idno>
                  <affiliation ref="#struct-194938"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Rawa</forename>
                    <surname>Abdallah</surname>
                  </persName>
                  <email type="md5">8ed789aa3e8d640e2528ecd0ca204bec</email>
                  <email type="domain">univ-rennes1.fr</email>
                  <idno type="idhal" notation="numeric">963653</idno>
                  <idno type="halauthorid" notation="string">847146-963653</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-4262-0382</idno>
                  <affiliation ref="#struct-303340"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Florence</forename>
                    <surname>Fourcade</surname>
                  </persName>
                  <idno type="halauthorid">699621-0</idno>
                  <affiliation ref="#struct-194938"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Abdeltif</forename>
                    <surname>Amrane</surname>
                  </persName>
                  <email type="md5">0d99a442446f0b47e7d42912f06bccde</email>
                  <email type="domain">univ-rennes1.fr</email>
                  <idno type="idhal" notation="string">abdeltif-amrane</idno>
                  <idno type="idhal" notation="numeric">768602</idno>
                  <idno type="halauthorid" notation="string">713977-768602</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-2622-2384</idno>
                  <idno type="IDREF">https://www.idref.fr/07491412X</idno>
                  <affiliation ref="#struct-194938"/>
                </author>
                <author role="crp">
                  <persName>
                    <forename type="first">Hayet</forename>
                    <surname>Djelal</surname>
                  </persName>
                  <email type="md5">070aca28bbec3cc5fc5de9941afff15d</email>
                  <email type="domain">unilasalle.fr</email>
                  <idno type="idhal" notation="string">hayet-djelal</idno>
                  <idno type="idhal" notation="numeric">1157029</idno>
                  <idno type="halauthorid" notation="string">704127-1157029</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-5619-2633</idno>
                  <idno type="GOOGLE SCHOLAR">https://scholar.google.fr/citations?user=CNTgCIgAAAAJ</idno>
                  <affiliation ref="#struct-1069231"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">22682</idno>
                <idno type="issn">0273-2289</idno>
                <title level="j">Applied Biochemistry and Biotechnology</title>
                <imprint>
                  <publisher>Humana Press</publisher>
                  <biblScope unit="volume">193</biblScope>
                  <biblScope unit="issue">9</biblScope>
                  <biblScope unit="pp">2872-2892</biblScope>
                  <date type="datePub">2021-09</date>
                  <date type="dateEpub">2021-05-03</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1007/s12010-021-03572-2</idno>
              <idno type="pubmed">33937964</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">Succinic acid</term>
                <term xml:lang="en">Sequential culture</term>
                <term xml:lang="en">Fermentation</term>
                <term xml:lang="en">Co-culture</term>
                <term xml:lang="en">Bio-based chemicals</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv.bio">Life Sciences [q-bio]/Biotechnology</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Microorganisms can produce a wide range of bio-based chemicals that can be used in various industrial applications as molecules of interest. In the present work, an analysis of the power production by pure culture, co-culture, and sequential culture was performed. In this study, both the mono-culture and the co-culture strategies of Actinobacillus succinogenes with Saccharomyces cerevisiae as carbon sources to produce succinic acid using glucose and fructose were examined. The cultures were performed in batch mode and a great attention was paid to the co-culture system to improve the biosynthetic pathway between A. succinogenes and S. cerevisiae by combining these two strains in a single fermentation process. Under microaerobic and anaerobic conditions, the process was characterized in terms of sugars concentration, cell density, metabolites, yield (mol-C products/ mol-C sugars), the temperature conditions for productivity, and pH. The results showed that the process could consume glucose and fructose and could adapt to different concentrations of the two sugars more quickly than by a single organism and the best results were obtained in a sequential co-culture recording 0.27 mol L(-1) of succinic acid concentration and a volumetric productivity of 0.3 g L(-1) h(-1). Under the investigated operating conditions, the combination of these two strains in a single reactor produced a significant amount of succinic acid (0.70 mol-C SA/mol-C substrates). A simultaneous and sequential co-culture strategy can be a powerful new approach in the field of bio-based chemical production.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-194938" status="VALID">
          <idno type="IdRef">161775241</idno>
          <idno type="ISNI">0000 0004 0385 6584</idno>
          <idno type="RNSR">200612270R</idno>
          <idno type="ROR">https://ror.org/00adwkx90</idno>
          <idno type="Wikidata">Q30261343</idno>
          <orgName>Institut des Sciences Chimiques de Rennes</orgName>
          <orgName type="acronym">ISCR</orgName>
          <date type="start">2006-01-01</date>
          <desc>
            <address>
              <addrLine>Campus de Beaulieu - Bât. 10 Avenue du Général Leclerc 35042 Rennes Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://iscr.univ-rennes.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-105160" type="direct"/>
            <relation active="#struct-117606" type="direct"/>
            <relation active="#struct-301232" type="indirect"/>
            <relation active="#struct-300373" type="direct"/>
            <relation active="#struct-341038" type="direct"/>
            <relation name="UMR6226" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-303340" status="VALID">
          <idno type="IdRef">08401430X</idno>
          <idno type="ISNI">0000 0001 2324 3572</idno>
          <idno type="ROR">https://ror.org/05x6qnc69</idno>
          <idno type="Wikidata">Q975461</idno>
          <orgName>الجامعة اللبنانية [بيروت] = Lebanese University [Beirut] = Université libanaise [Beyrouth]</orgName>
          <orgName type="acronym">LU / ULB</orgName>
          <date type="start">1951-01-01</date>
          <desc>
            <address>
              <addrLine>P.O. Box 6573/14 Badaro, Museum, Beirut</addrLine>
              <country key="LB"/>
            </address>
            <ref type="url">https://www.ul.edu.lb/</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-1069231" status="OLD">
          <idno type="ROR">https://ror.org/021wv9p83</idno>
          <orgName>UniLaSalle Rennes - Ecole des Métiers de l'Environnement</orgName>
          <date type="end">2025-12-31</date>
          <desc>
            <address>
              <addrLine>Campus de Ker Lann, Avenue Robert Schuman, 35170 Bruz</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://rennes.unilasalle.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-527123" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-105160" status="VALID">
          <idno type="IdRef">26693823X</idno>
          <idno type="ROR">https://ror.org/015m7wh34</idno>
          <orgName>Université de Rennes</orgName>
          <orgName type="acronym">UR</orgName>
          <desc>
            <address>
              <addrLine>Campus de Beaulieu, 263 avenue Général Leclerc, CS 74205, 35042 RENNES CEDEX</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.univ-rennes.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-117606" status="VALID">
          <idno type="ROR">https://ror.org/04xaa4j22</idno>
          <orgName>Institut National des Sciences Appliquées - Rennes</orgName>
          <orgName type="acronym">INSA Rennes</orgName>
          <desc>
            <address>
              <addrLine>20, avenue des Buttes de Coësmes - CS 70839 - 35708 Rennes cedex 7</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.insa-rennes.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-301232" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-301232" status="VALID">
          <idno type="IdRef">162105150</idno>
          <orgName>Institut National des Sciences Appliquées</orgName>
          <orgName type="acronym">INSA</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="institution" xml:id="struct-300373" status="VALID">
          <idno type="ROR">https://ror.org/01h0ffh48</idno>
          <orgName>Ecole Nationale Supérieure de Chimie de Rennes</orgName>
          <orgName type="acronym">ENSCR</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="institution" xml:id="struct-341038" status="VALID">
          <idno type="IdRef">232435103</idno>
          <idno type="ISNI">000000010626358X</idno>
          <idno type="ROR">https://ror.org/02cte4b68</idno>
          <orgName>Institut de Chimie - CNRS Chimie</orgName>
          <orgName type="acronym">INC-CNRS</orgName>
          <date type="start">2009-10-01</date>
          <desc>
            <address>
              <addrLine>CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE3 rue Michel-Ange75794 PARIS CEDEX 16 - France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.cnrs.fr/inc</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-527123" status="VALID">
          <idno type="IdRef">198281633</idno>
          <idno type="ROR">https://ror.org/05wy89733</idno>
          <orgName>UniLaSalle</orgName>
          <date type="start">2016-01-01</date>
          <desc>
            <address>
              <addrLine>19 rue Pierre Waguet – BP 30313 – 60026 BEAUVAIS cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.unilasalle.fr/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>