<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03219310</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-04-28T08:59:22+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Distinct oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand binding site</title>
            <author role="aut">
              <persName>
                <forename type="first">Florence</forename>
                <surname>Vincent</surname>
              </persName>
              <email type="md5">068043911d66cc8f14b3d98af4b40d64</email>
              <email type="domain">univ-amu.fr</email>
              <idno type="idhal" notation="string">florence-vincent</idno>
              <idno type="idhal" notation="numeric">742136</idno>
              <idno type="halauthorid" notation="string">12481-742136</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-8564-4184</idno>
              <idno type="IDREF">https://www.idref.fr/067202063</idno>
              <affiliation ref="#struct-482300"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Adam</forename>
                <surname>Round</surname>
              </persName>
              <idno type="halauthorid">875632-0</idno>
              <affiliation ref="#struct-2568"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Aline</forename>
                <surname>Reynaud</surname>
              </persName>
              <idno type="halauthorid">2203228-0</idno>
              <affiliation ref="#struct-199349"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Christophe</forename>
                <surname>Bordi</surname>
              </persName>
              <email type="md5">dced757d2d9d4d59b4109cf88490967b</email>
              <email type="domain">imm.cnrs.fr</email>
              <idno type="idhal" notation="string">christophe-bordi</idno>
              <idno type="idhal" notation="numeric">747508</idno>
              <idno type="halauthorid" notation="string">39910-747508</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-2877-6068</idno>
              <affiliation ref="#struct-472"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Alain</forename>
                <surname>Filloux</surname>
              </persName>
              <idno type="idhal" notation="numeric">756249</idno>
              <idno type="halauthorid" notation="string">134048-756249</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-1307-0289</idno>
              <affiliation ref="#struct-472"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Yves</forename>
                <surname>Bourne</surname>
              </persName>
              <email type="md5">a3b578e81497f9498e738a2f00e969ab</email>
              <email type="domain">univ-amu.fr</email>
              <idno type="idhal" notation="string">yves-bourne</idno>
              <idno type="idhal" notation="numeric">736930</idno>
              <idno type="halauthorid" notation="string">2540382-736930</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-3850-0548</idno>
              <idno type="IDREF">https://www.idref.fr/07075943X</idno>
              <affiliation ref="#struct-1157"/>
              <affiliation ref="#struct-199349"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Florence</forename>
                <surname>Vincent</surname>
              </persName>
              <email type="md5">068043911d66cc8f14b3d98af4b40d64</email>
              <email type="domain">univ-amu.fr</email>
            </editor>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2021-07-07 16:11:50</date>
              <date type="whenModified">2025-12-01 14:06:02</date>
              <date type="whenReleased">2021-07-07 16:11:50</date>
              <date type="whenProduced">2009</date>
              <ref type="externalLink" target="https://api.istex.fr/ark:/67375/WNG-LHGMBH5S-9/fulltext.pdf?sid=hal"/>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="649326">
                <persName>
                  <forename>Florence</forename>
                  <surname>Vincent</surname>
                </persName>
                <email type="md5">068043911d66cc8f14b3d98af4b40d64</email>
                <email type="domain">univ-amu.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03219310</idno>
            <idno type="halUri">https://hal.science/hal-03219310</idno>
            <idno type="halBibtex">vincent:hal-03219310</idno>
            <idno type="halRefHtml">&lt;i&gt;Environmental Microbiology&lt;/i&gt;, 2009, Pseudomonas, 12 (6), pp.1775 - 1786. &lt;a target="_blank" href="https://dx.doi.org/10.1111/j.1462-2920.2010.02264.x"&gt;&amp;#x27E8;10.1111/j.1462-2920.2010.02264.x&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Environmental Microbiology, 2009, Pseudomonas, 12 (6), pp.1775 - 1786. &amp;#x27E8;10.1111/j.1462-2920.2010.02264.x&amp;#x27E9;</idno>
            <availability status="restricted"/>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="ENSMP" corresp="PARISTECH">MINES ParisTech</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-AMU">Aix Marseille Université</idno>
            <idno type="stamp" n="INRA">INRA - Institut national de la recherche agronomique</idno>
            <idno type="stamp" n="ENSMP_MAT" corresp="ENSMP">Matériaux (MAT)</idno>
            <idno type="stamp" n="PARISTECH">ParisTech</idno>
            <idno type="stamp" n="PSL">Université Paris sciences et lettres</idno>
            <idno type="stamp" n="AGREENIUM">Archive ouverte en agrobiosciences</idno>
            <idno type="stamp" n="ENSMP_DEP_MM" corresp="ENSMP">Département Mécanique et Matériaux</idno>
            <idno type="stamp" n="ENSMP_DR" corresp="ENSMP">ENSMP_DR</idno>
            <idno type="stamp" n="INRAE">Institut National de Recherche en Agriculture, Alimentation et Environnement</idno>
            <idno type="stamp" n="ENSMP-PSL" corresp="PSL">MINES ParisTech - École nationale supérieure des mines de Paris - PSL</idno>
            <idno type="stamp" n="LISM-AMU">Laboratoire d'ingénierie des systèmes macromoléculaires</idno>
            <idno type="stamp" n="AFMB">Architecture et Fonction des Macromolécules Biologiques</idno>
            <idno type="stamp" n="FRM">Fondation pour la Recherche Médicale</idno>
            <idno type="stamp" n="INRAEPACA">INRAE PACA</idno>
            <idno type="stamp" n="ESRF"> European Synchrotron Radiation Facility</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Distinct oligomeric forms of the Pseudomonas aeruginosa RetS sensor domain modulate accessibility to the ligand binding site</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Florence</forename>
                    <surname>Vincent</surname>
                  </persName>
                  <email type="md5">068043911d66cc8f14b3d98af4b40d64</email>
                  <email type="domain">univ-amu.fr</email>
                  <idno type="idhal" notation="string">florence-vincent</idno>
                  <idno type="idhal" notation="numeric">742136</idno>
                  <idno type="halauthorid" notation="string">12481-742136</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-8564-4184</idno>
                  <idno type="IDREF">https://www.idref.fr/067202063</idno>
                  <affiliation ref="#struct-482300"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Adam</forename>
                    <surname>Round</surname>
                  </persName>
                  <idno type="halauthorid">875632-0</idno>
                  <affiliation ref="#struct-2568"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Aline</forename>
                    <surname>Reynaud</surname>
                  </persName>
                  <idno type="halauthorid">2203228-0</idno>
                  <affiliation ref="#struct-199349"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Christophe</forename>
                    <surname>Bordi</surname>
                  </persName>
                  <email type="md5">dced757d2d9d4d59b4109cf88490967b</email>
                  <email type="domain">imm.cnrs.fr</email>
                  <idno type="idhal" notation="string">christophe-bordi</idno>
                  <idno type="idhal" notation="numeric">747508</idno>
                  <idno type="halauthorid" notation="string">39910-747508</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-2877-6068</idno>
                  <affiliation ref="#struct-472"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Alain</forename>
                    <surname>Filloux</surname>
                  </persName>
                  <idno type="idhal" notation="numeric">756249</idno>
                  <idno type="halauthorid" notation="string">134048-756249</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-1307-0289</idno>
                  <affiliation ref="#struct-472"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Yves</forename>
                    <surname>Bourne</surname>
                  </persName>
                  <email type="md5">a3b578e81497f9498e738a2f00e969ab</email>
                  <email type="domain">univ-amu.fr</email>
                  <idno type="idhal" notation="string">yves-bourne</idno>
                  <idno type="idhal" notation="numeric">736930</idno>
                  <idno type="halauthorid" notation="string">2540382-736930</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-3850-0548</idno>
                  <idno type="IDREF">https://www.idref.fr/07075943X</idno>
                  <affiliation ref="#struct-1157"/>
                  <affiliation ref="#struct-199349"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">22298</idno>
                <idno type="issn">1462-2912</idno>
                <idno type="eissn">1462-2920</idno>
                <title level="j">Environmental Microbiology</title>
                <imprint>
                  <publisher>Society for Applied Microbiology and Wiley-Blackwell</publisher>
                  <biblScope unit="serie">Pseudomonas</biblScope>
                  <biblScope unit="volume">12</biblScope>
                  <biblScope unit="issue">6</biblScope>
                  <biblScope unit="pp">1775 - 1786</biblScope>
                  <date type="datePub">2009</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1111/j.1462-2920.2010.02264.x</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <classCode scheme="halDomain" n="sdv.mp.bac">Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology</classCode>
              <classCode scheme="halDomain" n="sdv.bbm.bs">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Bacterial two-component regulatory systems (TCSs) sense environmental stimuli to adapt the lifestyle of microbial populations. For many TCSs the stimulus is a ligand of unknown chemical nature. Pseudomonas aeruginosa utilizes the closely related RetS and LadS sensor kinases to switch between acute and chronic infections. These sensor proteins antagonistically mediate biofilm formation through communication with a central TCS, GacA/GacS. Recently, it was shown that RetS modulates the GacS sensor activity by forming RetS/GacS heterodimers. LadS and RetS are hybrid sensors with a signalling domain consisting of a 7-transmembrane (7TMR) region and a periplasmic sensor domain (diverse intracellular signalling module extracellular 2, DISMED2). The 2.65 Å resolution crystal structure of RetS DISMED2, called RetSp, reveals three distinct oligomeric states capable of domain swapping. The RetSp structure also displays two putative ligand binding sites. One is equivalent to the analogous site in the structurally-related carbohydrate binding module (CBM) but the second site is located at a dimer interface. These observations highlight the modular architecture and assembly of the RetSp fold and give clues on how homodimerization of RetS could be modulated upon ligand binding to control formation of a RetS/GacS heterodimer. Modelling the DISMED2 of LadS reveals conservation of only one ligand binding site, suggesting a distinct mechanism underlying the activity of this sensor kinase.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="laboratory" xml:id="struct-482300" status="OLD">
          <idno type="RNSR">200012177R</idno>
          <orgName>Architecture et fonction des Macromolécules Biologiques - UMR 6098</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2000</date>
          <date type="end">2011</date>
          <desc>
            <address>
              <addrLine>Marseille</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-92823" type="direct"/>
            <relation name="UMR6098" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-2568" status="OLD">
          <idno type="ROR">https://ror.org/02550n020</idno>
          <orgName>European Synchrotron Radiation Facility</orgName>
          <orgName type="acronym">ESRF</orgName>
          <date type="start">1988-01-01</date>
          <date type="end">2014-12-31</date>
          <desc>
            <address>
              <addrLine>6 rue Jules Horowitz BP220 38043 GRENOBLE CEDEX</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.esrf.fr/</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-199349" status="OLD">
          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2012-01-01</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <addrLine>Faculté des Sciences de Luminy - ESIL 163 avenue de Luminy CP 925 13288 MARSEILLE CEDEX 09</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>
            <relation name="USC1408" active="#struct-92114" type="direct"/>
            <relation name="UMR7257" active="#struct-198056" type="direct"/>
            <relation name="UMR7257" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-472" status="VALID">
          <idno type="IdRef">150500378</idno>
          <idno type="RNSR">201220358F</idno>
          <idno type="ROR">02hwc1z56</idno>
          <orgName>Laboratoire d'ingénierie des systèmes macromoléculaires</orgName>
          <orgName type="acronym">LISM</orgName>
          <date type="start">2012-01-01</date>
          <desc>
            <address>
              <addrLine>Batiment IBSM 31 Chemin Joseph Aiguier 13402 MARSEILLE CEDEX 20</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://lism.cnrs-mrs.fr</ref>
          </desc>
          <listRelation>
            <relation name="UMR7255" active="#struct-198056" type="direct"/>
            <relation name="UMR7255" active="#struct-303623" type="direct"/>
            <relation name="UMR7255 / UPR9027" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-1157" status="VALID">
          <idno type="IdRef">149799942</idno>
          <idno type="ISNI">0000 0004 0370 1427</idno>
          <idno type="RNSR">199719335J</idno>
          <idno type="ROR">https://ror.org/01q5ge486</idno>
          <orgName>Centre des Matériaux</orgName>
          <orgName type="acronym">MAT</orgName>
          <date type="start">1997-01-01</date>
          <desc>
            <address>
              <addrLine>Centre des matériaux - Campus de l'Innovation, Mines Paris-PSL, 21, allée des Marronniers, 78000 VERSAILLES SATORY -Anciennement Centre Pierre-Marie Fourt - 10 rue Henri Desbruères 91003 ÉVRY CEDEX</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.mat.minesparis.psl.eu/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-301492" type="direct"/>
            <relation active="#struct-564132" type="indirect"/>
            <relation name="UMR7633" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-92823" status="OLD">
          <idno type="IdRef">026403781</idno>
          <orgName>Université de Provence - Aix-Marseille 1</orgName>
          <date type="end">2011-12-31</date>
          <desc>
            <address>
              <addrLine>3, place Victor Hugo - 13331 Marseille Cedex 03</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-provence.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-92114" status="OLD">
          <idno type="ROR">https://ror.org/01x3gbx83</idno>
          <orgName>Institut National de la Recherche Agronomique</orgName>
          <orgName type="acronym">INRA</orgName>
          <date type="start">1946-05-18</date>
          <date type="end">2019-12-31</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inra.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-198056" status="VALID">
          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <idno type="ROR">https://ror.org/035xkbk20</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>
            <address>
              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-303623" status="VALID">
          <idno type="IdRef">026388278</idno>
          <idno type="ROR">https://ror.org/02vjkv261</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>
            <address>
              <addrLine>101, rue de Tolbiac, 75013 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-301492" status="VALID">
          <idno type="IdRef">026375249</idno>
          <idno type="ROR">https://ror.org/04y8cs423</idno>
          <orgName>Mines Paris - PSL (École nationale supérieure des mines de Paris)</orgName>
          <date type="start">1783-01-01</date>
          <desc>
            <address>
              <addrLine>60, boulevard Saint-Michel 75006 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.minesparis.psl.eu/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-564132" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-564132" status="VALID">
          <idno type="IdRef">241597595</idno>
          <idno type="ISNI">0000 0004 1784 3645</idno>
          <idno type="ROR">https://ror.org/013cjyk83</idno>
          <orgName>Université Paris Sciences et Lettres</orgName>
          <orgName type="acronym">PSL</orgName>
          <desc>
            <address>
              <addrLine>60 rue Mazarine 75006 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.psl.eu/</ref>
          </desc>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>