Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy
Résumé
Functionalization of AFM probes with biomolecules or microorganisms allows for a better understanding of the interaction mechanisms driving microbial adhesion. Here we describe the most commonly used protocols to graft molecules and bacteria to AFM cantilevers. The bioprobes obtained that way enable to measure forces down to the single-cell and single-molecule levels.
Domaines
Bactériologie
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Book chapter - El-Kirat-Chatel Beaussart - HAL.pdf (572.33 Ko)
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