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Autre Publication Scientifique Année : 2018

Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy

Résumé

Functionalization of AFM probes with biomolecules or microorganisms allows for a better understanding of the interaction mechanisms driving microbial adhesion. Here we describe the most commonly used protocols to graft molecules and bacteria to AFM cantilevers. The bioprobes obtained that way enable to measure forces down to the single-cell and single-molecule levels.

Domaines

Bactériologie
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Dates et versions

hal-03215692 , version 1 (27-06-2022)

Identifiants

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Sofiane El-Kirat-Chatel, Audrey Beaussart. Probing Bacterial Adhesion at the Single-Molecule and Single-Cell Levels by AFM-Based Force Spectroscopy. Nanoscale Imaging : Methods and Protocols, 2018, pp.403-414. ⟨10.1007/978-1-4939-8591-3_24⟩. ⟨hal-03215692⟩
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