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Communication Dans Un Congrès Année : 2012

Nonlinear microbubble behaviour for enhanced drug uptake

Résumé

The purpose of this study was to investigate the physical mechanisms of sonoporation, to understand and ameliorate ultrasound-assisted drug and gene delivery. Sonoporation is the transient permeabilisation of a cell membrane with the help of ultrasound and/or an ultra- sound contrast agent, allowing for the trans-membrane delivery and cellular uptake of macro- molecules between 10 kDa and 3 MDa. We studied the behaviour of ultrasound contrast agent microbubble near cancer cells at low acoustic amplitudes. After administering an ultrasound contrast agent, HeLa cells were sub- jected to 6.6-MHz ultrasound with a mechanical index of 0.2 and observed using a high-speed camera. Microbubbles were seen to enter cells and rapidly dissolve. The quick dissolution after enter- ing suggests that the microbubbles lose (part of) their shell whilst entering. We have demonstrated that lipid-shelled microbubbles can be forced to enter cells at a low acoustic amplitude. Hence, if a therapeutic load is added to the bubble, ultrasound-guided de- livery could be facilitated at diagnostic settings. However, these results may have implications for the safety regulations on the use of ultrasound contrast agents for diagnostic imaging.
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Dates et versions

hal-03195601 , version 1 (11-04-2021)

Identifiants

  • HAL Id : hal-03195601 , version 1

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Spiros Kotopoulis, Anthony Delalande, Chantal Pichon, Michiel Postema. Nonlinear microbubble behaviour for enhanced drug uptake. 19th International Congress on Sound and Vibration, Vilnius, Lithuania, July 8–12, 2012, Jul 2012, Vilnius, Lithuania. pp.685. ⟨hal-03195601⟩
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