Developing a MEMS device for high-throughput multi-parameter single cell biophysical analysis
Quentin Rezard
(1, 2)
,
Gregoire Perret
(2)
,
Jean Claude Gerbedoen
(2)
,
Deniz Pekin
(3, 2)
,
Fabrizio Cleri
(1, 4)
,
Dominique Collard
(2)
,
Chann Lagadec
(5)
,
Mehmet Tarhan
(1)
1
IEMN -
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
2 LIMMS - Laboratory for Integrated Micro Mechatronics Systems
3 LilNCog - Lille Neurosciences & Cognition - U 1172
4 PHYSIQUE - IEMN - Physique - IEMN
5 CANTHER - Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277
2 LIMMS - Laboratory for Integrated Micro Mechatronics Systems
3 LilNCog - Lille Neurosciences & Cognition - U 1172
4 PHYSIQUE - IEMN - Physique - IEMN
5 CANTHER - Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277
Jean Claude Gerbedoen
- Fonction : Auteur
- PersonId : 753136
- IdHAL : jean-claude-gerbedoen
- ORCID : 0000-0001-5954-0616
Deniz Pekin
- Fonction : Auteur
- PersonId : 965517
Fabrizio Cleri
- Fonction : Auteur
- PersonId : 748019
- IdHAL : fabrizio-cleri
- ORCID : 0000-0003-0272-7441
- IdRef : 168047853
Dominique Collard
- Fonction : Auteur
- PersonId : 770196
- ORCID : 0000-0003-3244-1436
- IdRef : 070432147
Chann Lagadec
- Fonction : Auteur
- PersonId : 1066377
- IdHAL : chann-lagadec
Mehmet Tarhan
Connectez-vous pour contacter l'auteur
- Fonction : Auteur correspondant
- PersonId : 748912
- IdHAL : mehmet-cagatay-tarhan
Connectez-vous pour contacter l'auteur
Résumé
We introduced a MEMS device to perform direct measurements on electrical and mechanical properties of single cancer cells. Working in a continuous flow format permits high-throughput measurements. The device design allows us to define fixed or movable electrodes as part of the side-walls of an embedded microchannel. The stable air-liquid interface at micro-sized gaps between the electrodes and the channel wall provide flow continuity without causing any leakage problems. This unified processing approach allows us to define optimized electrode pattern configuration for both electrical and mechanical characterization areas. Moreover, the movable electrode is in direct contact with passing cells which enables direct sensing during compression. The device can perform both electrical and mechanical measurements on single cells in a continuous flow as demonstrated and also validated by optical control measurements. The proposed method aims at providing high-throughput biophysical cytometry, in an optics- and marker-free way, for cancer cell evaluation.
Format du dépôt | Fichier |
---|---|
Type de dépôt | Communication dans un congrès |
Titre |
en
Developing a MEMS device for high-throughput multi-parameter single cell biophysical analysis
|
Résumé |
en
We introduced a MEMS device to perform direct measurements on electrical and mechanical properties of single cancer cells. Working in a continuous flow format permits high-throughput measurements. The device design allows us to define fixed or movable electrodes as part of the side-walls of an embedded microchannel. The stable air-liquid interface at micro-sized gaps between the electrodes and the channel wall provide flow continuity without causing any leakage problems. This unified processing approach allows us to define optimized electrode pattern configuration for both electrical and mechanical characterization areas. Moreover, the movable electrode is in direct contact with passing cells which enables direct sensing during compression. The device can perform both electrical and mechanical measurements on single cells in a continuous flow as demonstrated and also validated by optical control measurements. The proposed method aims at providing high-throughput biophysical cytometry, in an optics- and marker-free way, for cancer cell evaluation.
|
Auteur(s) |
Quentin Rezard
1, 2
, Gregoire Perret
2
, Jean Claude Gerbedoen
2
, Deniz Pekin
3, 2
, Fabrizio Cleri
1, 4
, Dominique Collard
2
, Chann Lagadec
5
, Mehmet Tarhan
1
1
IEMN -
Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520
( 1066983 )
- [Univ. Lille, CNRS, Centrale Lille Institut, Junia, Univ. Polytechnique Hauts-de-France] ––
Laboratoire Central – Cité Scientifique – Avenue Poincaré – CS 60069 – 59652 VILLENEUVE D’ASCQ CEDEX
- France
2
LIMMS -
Laboratory for Integrated Micro Mechatronics Systems
( 2208 )
- LIMMS/CNRS-IIS(UMI 2820)/ Institute of Industrial Science, University of Tokyo/ 4-6-1 Komaba, Meguro-ku, Tokyo 153-8505 Japan/
- Japon
3
LilNCog -
Lille Neurosciences & Cognition - U 1172
( 1049721 )
- (ex-JPARC) --
Université de Lille
- Faculté de Médecine - Bâtiment Biserte
- 1 place de Verdun
- 59045 Lille Cedex
- Tél : +33 (0)3 20 29 88 50
- France
4
PHYSIQUE - IEMN -
Physique - IEMN
( 1067373 )
- Groupe Physique - (Physics group) - IEMN UMR8520
- France
5
CANTHER -
Cancer Heterogeneity, Plasticity and Resistance to Therapies - UMR 9020 - U 1277
( 1004619 )
- Laboratoire CANTHER, UMR 9020 CNRS-1277 Inserm --
Hétérogénéité, Plasticité et Résistance aux Thérapies des Cancers -
Université de Lille - Bâtiment Cancer - Place de Verdun -
59000 LILLE
- France
|
Langue du document |
Anglais
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Invité |
Non
|
Audience |
Internationale
|
Actes |
Oui
|
Titre de la collection |
Proceedings of the IEEE 34th International Conference on Micro Electro Mechanical Systems, MEMS 2021
|
Numéro |
ISBN 978-166541912-3 ; e-ISBN 978-1-6654-1912-3
|
Page/Identifiant |
494-497
|
Titre du congrès |
2021 IEEE 34th International Conference on Micro Electro Mechanical Systems, MEMS 2021
|
Date début congrès |
2021-01-25
|
Date fin congrès |
2021-01-29
|
Ville |
Gainesville (on line)
|
Pays |
États-Unis
|
Domaine(s) |
|
Éditeur commercial |
|
Référence interne |
|
Mots-clés |
en
Biophysical cell characterization, high-throughput measurements, single-cell analysis, cancer
|
DOI | 10.1109/MEMS51782.2021.9375176 |
UT key WOS | 000667731600120 |
Fichier principal
Rezard_2021_Developing_A_Mems_Device_for_High-Throughput_Multi-Parameter_Single_Cell_Biophysical_Analysis.pdf ( 1.01 Mo
)
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