Sonoporation at a low mechanical index - Archive ouverte HAL
Article Dans Une Revue Bubble Science, Engineering & Technology Année : 2011

Sonoporation at a low mechanical index

Résumé

Purpose: The purpose of this study was to investigate the physical mechanisms of sonoporation, in order to understand and improve ultrasound-assisted drug and gene delivery. Sonoporation is the transient permeabilisation and resealing of a cell membrane with the help of ultrasound and/or an ultrasound contrast agent, allowing for the trans-membrane delivery and cellular uptake of macromolecules between 10 kDa and 3 MDa. Methods: We studied the behaviour of ultrasound contrast agent microbubbles near cancer cells at low acoustic amplitudes. After administering an ultrasound contrast agent, HeLa cells were subjected to 6.6-MHz ultrasound with a mechanical index of 0.2 and observed with a high-speed camera. Results: Microbubbles were seen to enter cells and rapidly dissolve. The quick dissolution after entering suggests that the microbubbles lose (part of) their shell whilst entering. Conclusions: We have demonstrated that lipid-shelled microbubbles can be forced to enter cells at a low mechanical index. Hence, if a therapeutic agent is added to the shell of the bubble or inside the bubble, ultrasound-guided delivery could be facilitated at diagnostic settings. In addition, these results may have implications for the safety regulations on the use of ultrasound contrast agents for diagnostic imaging.
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Dates et versions

hal-03186064 , version 1 (30-03-2021)

Identifiants

  • HAL Id : hal-03186064 , version 1

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Anthony Delalande, Spiros Kotopoulis, Tijs Rovers, Chantal Pichon, Michiel Postema. Sonoporation at a low mechanical index. Bubble Science, Engineering & Technology, 2011, 3 (1), pp.3-12. ⟨hal-03186064⟩
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