Football, as the most popular sport in the world, has a very wide spread world-wide. With the wide spread of football, more and more people participate in foot-ball activities. Football is a very strenuous sport and athletes are easily injured. This article examines the problem of kicking through two experiments, one is the normal kicking process, and the other is the process of imitating the kicking. The two sets of experiments use the same motions, but their applied external forces are different. In the normal kicking, the football will receive a force on the foot during the kicking process. Conversely, in the imitation kicking, the foot is not in contact with the football and no extra force is apply on the foot. Analyze the im-pact of football on the body by comparing the above two experiments. OpenSim software is used to calculate the forces of each muscle by simulating the process of kicking a ball to analyze the muscle fatigue. It is judged that those muscles are vulnerable to injury during the kick. Athletes should focus on strengthening the exercise of these muscles, increase the strength of related muscles, and focus on protecting related muscles during exercise to reduce injuries.