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Chapitre D'ouvrage Année : 2016

Cellular Localization of Engineered Human LINE-1 RNA and Proteins

Résumé

The human LINE-1 retrotransposon has the ability to mobilize into a new genomic location through an intracellular replication cycle. Immunofluorescence and in situ hybridization experiments have been developed to detect subcellular localization of retrotransposition intermediates (i.e., ORF1p, ORF2p, and L1 mRNA). Currently, these protocols are also used to validate the interaction between retrotransposition complex components and potential cellular partners involved in L1 replication. Here, we describe in details methods for the identification of LINE-1 proteins and/or RNA in cells transfected with vectors expressing engineered human LINE-1 elements.
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Dates et versions

hal-03172376 , version 1 (17-03-2021)

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Aurélien J. Doucet, Eugenia Basyuk, Nicolas Gilbert. Cellular Localization of Engineered Human LINE-1 RNA and Proteins. Jose L. Garcia-Pérez. Transposons and Retrotransposons. Methods in Molecular Biology, 1400, Humana Press, pp.281-297, 2016, Print ISBN 978-1-4939-3370-9 Online ISBN 978-1-4939-3372-3. ⟨10.1007/978-1-4939-3372-3_18⟩. ⟨hal-03172376⟩
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