Sedimentation Velocity Methods for the Characterization of Protein Heterogeneity and Protein Affinity Interactions - Archive ouverte HAL Access content directly
Journal Articles Methods in Molecular Biology Year : 2021

Sedimentation Velocity Methods for the Characterization of Protein Heterogeneity and Protein Affinity Interactions

Abstract

Sedimentation velocity analytical ultracentrifugation is a powerful and versatile tool for the characterization of proteins and macromolecular complexes in solution. The direct modeling of the sedimentation process using modern computational strategies allows among others to assess the homogeneity/heterogeneity state of protein samples and to characterize protein associations. In this chapter, we will provide theoretical backgrounds and protocols to analyze the size distribution of protein samples and to determine the affinity of protein-protein hetero-associations.
No file

Dates and versions

hal-03163021 , version 1 (09-03-2021)

Identifiers

Cite

Christine Ebel, Catherine Birck. Sedimentation Velocity Methods for the Characterization of Protein Heterogeneity and Protein Affinity Interactions. Methods in Molecular Biology, 2021, 2247, pp.155-171. ⟨10.1007/978-1-0716-1126-5_9⟩. ⟨hal-03163021⟩
83 View
0 Download

Altmetric

Share

Gmail Facebook X LinkedIn More