Leather industry plays an essential role in the world’s economy; however, it also has a negative environmental impact due to the generation of significant quantities of wastes, some of which are classified as hazardous chemicals. Chrome tanning, the most popular tanning process, employs chromium salts, acids, and some other chemicals. Some dyes can be also a source of chromium. As a result, hexavalent chromium, a known carcinogenic and mutagenic, can be found in leather products and cause allergic dermatitis or trigger other diseases. For this reason, it is important to quantify the total amount of chromium in final leather goods, as well as the oxidation state in which this element is found. This paper aims to summarize chromium contamination due to the leather production processes, and to review the analytical methods that have been used to determine chromium’s most abundant species: Cr(III) and Cr(VI) in leather and other matrices (foodstuffs, cosmetic products, environmental, and pharmaceutical samples). The international and European regulations are presented as well as the last academic developments to extract and quantify chromium species. The future outlook of pretreatment and quantification techniques are also discussed in this work, with a special focus on chromium interconversions.