Exploitation of β -glycosyl azides for the preparation of α -glycosynthases - Archive ouverte HAL Access content directly
Journal Articles Biocatalysis and Biotransformation Year : 2012

Exploitation of β -glycosyl azides for the preparation of α -glycosynthases

Beatrice Cobucci-Ponzano
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Carmela Zorzetti
  • Function : Author
Andrea Strazzulli
  • Function : Author
Emiliano Bedini
  • Function : Author
Maria Michela Corsaro
  • Function : Author
Mos È Rossi
  • Function : Author
Marco Moracci

Abstract

An interesting approach for the chemo-enzymatic synthesis of carbohydrates is the use of glycosynthases, a class of mutant glycosidases derived from β-glycoside hydrolases obtained by replacement of the active site nucleophile with a non-nucleophilic residue. However, the scarcity of α-glycosynthases has so far hampered access to the synthesis of a large class of oligosaccharides of biotechnological interest. We review here a new glycosynthetic methodology for the production of two retaining α-fucosynthases and an α-galactosynthase exploiting β-glycosyl azide derivatives. The general applicability of this approach, which opens new perspectives in the use of azide derivatives for the production of novel α-glycosynthases, is discussed.
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Dates and versions

hal-03160483 , version 1 (25-03-2021)

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Beatrice Cobucci-Ponzano, Carmela Zorzetti, Andrea Strazzulli, Emiliano Bedini, Maria Michela Corsaro, et al.. Exploitation of β -glycosyl azides for the preparation of α -glycosynthases. Biocatalysis and Biotransformation, 2012, ⟨10.3109/10242422.2012.679814⟩. ⟨hal-03160483⟩
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