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Article Dans Une Revue Physical Review X Année : 2021

Cell and Nucleus Shape as an Indicator of Tissue Fluidity in Carcinoma

Steffen Grosser
  • Fonction : Auteur
Jürgen Lippoldt
  • Fonction : Auteur
Linda Oswald
  • Fonction : Auteur
Daniel M Sussman
  • Fonction : Auteur
Frédéric Renner
  • Fonction : Auteur
Pablo Gottheil
  • Fonction : Auteur
Erik W Morawetz
  • Fonction : Auteur
Thomas Fuhs
  • Fonction : Auteur
Xiaofan Xie
  • Fonction : Auteur
Steve Pawlizak
  • Fonction : Auteur
Anatol W Fritsch
  • Fonction : Auteur
Benjamin Wolf
  • Fonction : Auteur
Lars-Christian Horn
  • Fonction : Auteur
Susanne Briest
  • Fonction : Auteur
Bahriye Aktas
  • Fonction : Auteur
M Lisa Manning
  • Fonction : Auteur
Josef A Käs
  • Fonction : Auteur

Résumé

Tissue, cell, and nucleus morphology change during tumor progression. In 2D confluent cell cultures, different tissue states, such as fluid (unjammed) and solid (jammed), are correlated with cell shapes. These results do not have to apply a priori to three dimensions. Cancer cell motility requires and corresponds to a fluidization of the tumor tissue on the bulk level. Here, we investigate bulk tissue fluidity in 3D and determine how it correlates with cell and nucleus shape. In patient samples of mamma and cervix carcinoma, we find areas where cells can move or are immobile. We compare 3D cell spheroids composed of cells from a cancerous and a noncancerous cell line. Through bulk mechanical spheroid-fusion experiments and single live-cell tracking, we show that the cancerous sample is fluidized by active cells moving through the tissue. The healthy, epithelial sample with immobile cells behaves more solidlike. 3D segmentations of the samples show that the degree of tissue fluidity correlates with elongated cell and nucleus shapes. This correlation links cell shapes to cell motility and bulk mechanical behavior. We find two active states of matter in solid tumors: an amorphous glasslike state with characteristics of 3D cell jamming and a disordered fluid state. Individual cell and nucleus shape may serve as a marker for metastatic potential to foster personalized cancer treatment.
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Dates et versions

hal-03158879 , version 1 (04-03-2021)

Identifiants

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Steffen Grosser, Jürgen Lippoldt, Linda Oswald, Matthias Merkel, Daniel M Sussman, et al.. Cell and Nucleus Shape as an Indicator of Tissue Fluidity in Carcinoma. Physical Review X, 2021, 11 (1), pp.011033. ⟨10.1103/PhysRevX.11.011033⟩. ⟨hal-03158879⟩
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