The evolution of flow structures downstream a single pair of delta-winglet vortex generators (VGs) is investigated experimentally using stereoscopic particle image velocimetry. In addition, the laser Doppler anemometer technique is performed to characterize the upstream flow. Experiments are conducted in a bounded channel flow (height H) for the Reynolds numbers (ReDH, based on the hydraulic diameter height) ranging from 400 to 12 000. The purpose of this study is to provide detail insight into the generation and the dissipation of longitudinal vortices over a wide flow regime range including the laminar–turbulent transition. With a focus on transverse sections, the flow field is detailed. For all flow regimes, the main flow topology shows that the two main counter-rotating vortices are generated at a certain streamwise distance downstream the VG and then are advected gradually toward the channel lateral-walls. A secondary vortex pair is induced closer to the wall. Our results show that close to the VGs, local regions (1 > z/H > −1) are strongly defined as the inception of the turbulence production. The intensity of this latter is shown to vanish beyond a certain distance far from the origin of the perturbation (when x/H is greater than 3). The instantaneous flow structure describes the mechanism of vortex generation, relying on the intermittence of the flow organization and the sweep and ejection event balance. Detailed analysis on the turbulence properties and wall shear stress has been assessed and revealed that the flow transition induced by the perturbation of the VG is achieved at a Reynolds number no greater than 1500