<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03150854</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-15T12:02:05+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">Characterization of three bacterial glycoside hydrolase family 9 endoglucanases with different modular architectures isolated from a compost metagenome</title>
            <author role="aut">
              <persName>
                <forename type="first">Laure</forename>
                <surname>Aymé</surname>
              </persName>
              <idno type="halauthorid">946746-0</idno>
              <affiliation ref="#struct-300006"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Agnès</forename>
                <surname>Hébert</surname>
              </persName>
              <idno type="halauthorid">522987-0</idno>
              <affiliation ref="#struct-300006"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Bernard</forename>
                <surname>Henrissat</surname>
              </persName>
              <email type="md5">7464b6a2c6556684b4def860c311c47a</email>
              <email type="domain">afmb.univ-mrs.fr</email>
              <idno type="idhal" notation="numeric">833340</idno>
              <idno type="halauthorid" notation="string">141138-833340</idno>
              <affiliation ref="#struct-1002356"/>
              <affiliation ref="#struct-422761"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Vincent</forename>
                <surname>Lombard</surname>
              </persName>
              <email type="md5">5e168cb5db53a19959c0b2a90b652886</email>
              <email type="domain">univ-amu.fr</email>
              <idno type="idhal" notation="string">vincent-lombard</idno>
              <idno type="idhal" notation="numeric">751357</idno>
              <idno type="halauthorid" notation="string">2338711-751357</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-9684-7067</idno>
              <affiliation ref="#struct-1002356"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Nathalie</forename>
                <surname>Franche</surname>
              </persName>
              <idno type="halauthorid">927041-0</idno>
              <affiliation ref="#struct-198056"/>
              <affiliation ref="#struct-479"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Stéphanie</forename>
                <surname>Perret</surname>
              </persName>
              <idno type="halauthorid">1669491-0</idno>
              <affiliation ref="#struct-198056"/>
              <affiliation ref="#struct-479"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Etienne</forename>
                <surname>Jourdier</surname>
              </persName>
              <email type="md5">13441d77b48a0a5ab4bf18db1dfddb37</email>
              <email type="domain">ifpen.fr</email>
              <idno type="idhal" notation="string">etienne-jourdier</idno>
              <idno type="idhal" notation="numeric">948512</idno>
              <idno type="halauthorid" notation="string">778958-948512</idno>
              <idno type="IDREF">https://www.idref.fr/167527185</idno>
              <idno type="ORCID">https://orcid.org/0000-0003-3502-2982</idno>
              <affiliation ref="#struct-300006"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Senta</forename>
                <surname>Heiss-Blanquet</surname>
              </persName>
              <email type="md5">0d2fce204745e0c6d984f4d0a892b065</email>
              <email type="domain">ifpen.fr</email>
              <idno type="idhal" notation="numeric">1000234</idno>
              <idno type="halauthorid" notation="string">850838-1000234</idno>
              <affiliation ref="#struct-300006"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>Catherine</forename>
                <surname>Belli</surname>
              </persName>
              <email type="md5">a47db6c3bb6e09822cde2430c3a6d16a</email>
              <email type="domain">ifpen.fr</email>
            </editor>
            <funder ref="#projanr-35044"/>
            <funder>This work was supported by Agence Nationale de Recherche Grant number: ANR-12-BIO-ME-006-01</funder>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2021-03-05 12:56:17</date>
              <date type="whenWritten">2020-07-07</date>
              <date type="whenModified">2026-04-23 18:18:02</date>
              <date type="whenReleased">2021-03-05 17:31:19</date>
              <date type="whenProduced">2021-05</date>
              <date type="whenEndEmbargoed">2023-01-15</date>
              <ref type="file" target="https://ifp.hal.science/hal-03150854v1/document">
                <date notBefore="2023-01-15"/>
              </ref>
              <ref type="file" subtype="author" n="1" target="https://ifp.hal.science/hal-03150854v1/file/Characterization%20of%20Three%20Bacterial%20Glycoside.pdf" id="file-3150854-2771557">
                <date notBefore="2023-01-15"/>
              </ref>
              <ref type="annex" n="0" target="https://ifp.hal.science/hal-03150854v1/file/Characterization%20of%20Three%20Bacterial%20Glycoside%20Sup%20Mat%203%20%282%29.pdf" id="file-3150854-2771558">
                <date notBefore="2021-03-05"/>
              </ref>
              <ref type="annex" n="0" target="https://ifp.hal.science/hal-03150854v1/file/Characterization%20of%20Three%20Bacterial%20Glycoside%20Sup%20Mat%205%20%281%29.xlsx" id="file-3150854-2771559">
                <date notBefore="2021-03-05"/>
              </ref>
              <ref type="annex" n="0" target="https://ifp.hal.science/hal-03150854v1/file/Characterization%20of%20Three%20Bacterial%20Sup%20Mat.pdf" id="file-3150854-2771560">
                <date notBefore="2021-03-05"/>
              </ref>
              <ref type="externalLink" target="https://hal-ifp.archives-ouvertes.fr/hal-03150854/file/Characterization%20of%20Three%20Bacterial%20Glycoside%20Sup%20Mat%203%20%282%29.pdf"/>
              <fs>
                <f name="inra_etatDocument_local" notation="numeric">
                  <numeric>0</numeric>
                </f>
                <f name="inra_etatDocument_local" notation="string" n="0">
                  <string>inra_etatDocument_local_0</string>
                </f>
                <f name="inra_publicVise_local" notation="string" n="SC">
                  <string>inra_publicVise_local_SC</string>
                </f>
              </fs>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="656385">
                <persName>
                  <forename>Catherine</forename>
                  <surname>Belli</surname>
                </persName>
                <email type="md5">a47db6c3bb6e09822cde2430c3a6d16a</email>
                <email type="domain">ifpen.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03150854</idno>
            <idno type="halUri">https://ifp.hal.science/hal-03150854</idno>
            <idno type="halBibtex">ayme:hal-03150854</idno>
            <idno type="halRefHtml">&lt;i&gt;Biochimica et Biophysica Acta (BBA) - General Subjects&lt;/i&gt;, 2021, 1865 (5), pp.129848. &lt;a target="_blank" href="https://dx.doi.org/10.1016/j.bbagen.2021.129848"&gt;&amp;#x27E8;10.1016/j.bbagen.2021.129848&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Biochimica et Biophysica Acta (BBA) - General Subjects, 2021, 1865 (5), pp.129848. &amp;#x27E8;10.1016/j.bbagen.2021.129848&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://about.hal.science/hal-authorisation-v1/">HAL Authorization<ref corresp="#file-3150854-2771557"/><ref corresp="#file-3150854-2771558"/><ref corresp="#file-3150854-2771559"/><ref corresp="#file-3150854-2771560"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="SDE">Sciences De l'Environnement</idno>
            <idno type="stamp" n="IFP">IFP Energies Nouvelles</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="UNIV-AMU">Aix Marseille Université</idno>
            <idno type="stamp" n="GIP-BE">GIP Bretagne Environnement</idno>
            <idno type="stamp" n="INRAE">Institut National de Recherche en Agriculture, Alimentation et Environnement</idno>
            <idno type="stamp" n="TEST-HALCNRS">Collection test HAL CNRS</idno>
            <idno type="stamp" n="ANR">ANR</idno>
            <idno type="stamp" n="AFMB">Architecture et Fonction des Macromolécules Biologiques</idno>
            <idno type="stamp" n="MICA-UNITES">MICA-Unités </idno>
            <idno type="stamp" n="RESEAU-EAU">Réseau "Systèmes Agricoles et Eau"</idno>
            <idno type="stamp" n="INRAEPACA">INRAE PACA</idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">Characterization of three bacterial glycoside hydrolase family 9 endoglucanases with different modular architectures isolated from a compost metagenome</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Laure</forename>
                    <surname>Aymé</surname>
                  </persName>
                  <idno type="halauthorid">946746-0</idno>
                  <affiliation ref="#struct-300006"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Agnès</forename>
                    <surname>Hébert</surname>
                  </persName>
                  <idno type="halauthorid">522987-0</idno>
                  <affiliation ref="#struct-300006"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Bernard</forename>
                    <surname>Henrissat</surname>
                  </persName>
                  <email type="md5">7464b6a2c6556684b4def860c311c47a</email>
                  <email type="domain">afmb.univ-mrs.fr</email>
                  <idno type="idhal" notation="numeric">833340</idno>
                  <idno type="halauthorid" notation="string">141138-833340</idno>
                  <affiliation ref="#struct-1002356"/>
                  <affiliation ref="#struct-422761"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Vincent</forename>
                    <surname>Lombard</surname>
                  </persName>
                  <email type="md5">5e168cb5db53a19959c0b2a90b652886</email>
                  <email type="domain">univ-amu.fr</email>
                  <idno type="idhal" notation="string">vincent-lombard</idno>
                  <idno type="idhal" notation="numeric">751357</idno>
                  <idno type="halauthorid" notation="string">2338711-751357</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-9684-7067</idno>
                  <affiliation ref="#struct-1002356"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Nathalie</forename>
                    <surname>Franche</surname>
                  </persName>
                  <idno type="halauthorid">927041-0</idno>
                  <affiliation ref="#struct-198056"/>
                  <affiliation ref="#struct-479"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Stéphanie</forename>
                    <surname>Perret</surname>
                  </persName>
                  <idno type="halauthorid">1669491-0</idno>
                  <affiliation ref="#struct-198056"/>
                  <affiliation ref="#struct-479"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Etienne</forename>
                    <surname>Jourdier</surname>
                  </persName>
                  <email type="md5">13441d77b48a0a5ab4bf18db1dfddb37</email>
                  <email type="domain">ifpen.fr</email>
                  <idno type="idhal" notation="string">etienne-jourdier</idno>
                  <idno type="idhal" notation="numeric">948512</idno>
                  <idno type="halauthorid" notation="string">778958-948512</idno>
                  <idno type="IDREF">https://www.idref.fr/167527185</idno>
                  <idno type="ORCID">https://orcid.org/0000-0003-3502-2982</idno>
                  <affiliation ref="#struct-300006"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Senta</forename>
                    <surname>Heiss-Blanquet</surname>
                  </persName>
                  <email type="md5">0d2fce204745e0c6d984f4d0a892b065</email>
                  <email type="domain">ifpen.fr</email>
                  <idno type="idhal" notation="numeric">1000234</idno>
                  <idno type="halauthorid" notation="string">850838-1000234</idno>
                  <affiliation ref="#struct-300006"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">11115</idno>
                <idno type="issn">0304-4165</idno>
                <idno type="eissn">1872-8006</idno>
                <title level="j">Biochimica et Biophysica Acta (BBA) - General Subjects</title>
                <imprint>
                  <publisher>Elsevier</publisher>
                  <biblScope unit="volume">1865</biblScope>
                  <biblScope unit="issue">5</biblScope>
                  <biblScope unit="pp">129848</biblScope>
                  <date type="datePub">2021-05</date>
                  <date type="dateEpub">2021-01-15</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1016/j.bbagen.2021.129848</idno>
              <idno type="pubmed">33495646</idno>
              <idno type="wos">000611481100004</idno>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">metagenome</term>
                <term xml:lang="en">cellulose hydrolysis</term>
                <term xml:lang="en">endoglucanase</term>
                <term xml:lang="en">glycoside hydrolase</term>
              </keywords>
              <classCode scheme="halDomain" n="sdv">Life Sciences [q-bio]</classCode>
              <classCode scheme="halDomain" n="sde">Environmental Sciences</classCode>
              <classCode scheme="vocinrae" n="c_5875">glycoside hydrolase</classCode>
              <classCode scheme="vocinrae" n="c_8748">endoglucanase</classCode>
              <classCode scheme="vocinrae" n="c_8245">cellulose</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Background: Environmental bacteria express a wide diversity of glycoside hydrolases (GH). Screening and characterization of GH from metagenomic sources provides an insight into biomass degradation strategies of non-cultivated prokaryotes. Methods: In the present report, we screened a compost metagenome for lignocellulolytic activities and identified six genes encoding enzymes belonging to family GH9 (GH9a-f). Three of these enzymes (GH9b, GH9d and GH9e) were successfully expressed and characterized. Results: A phylogenetic analysis of the catalytic domain of pro-and eukaryotic GH9 enzymes suggested the existence of two major subgroups. Bacterial GH9s displayed a wide variety of modular architectures and those harboring an N-terminal Ig-like domain, such as GH9b and GH9d, segregated from the remainder. We purified and characterized GH9 endoglucanases from both subgroups and examined their stabilities, substrate specificities and product profiles. GH9e exhibited an original hydrolysis pattern, liberating an elevated proportion of oligosaccharides longer than cellobiose. All of the enzymes exhibited processive behavior and a synergistic action on crystalline cellulose. Synergy was also evidenced between GH9d and a GH48 enzyme identified from the same metagenome. Conclusions: The characterized GH9 enzymes displayed different modular architectures and distinct substrate and product profiles. The presence of a cellulose binding domain was shown to be necessary for binding and digestion of insoluble cellulosic substrates, but not for processivity. General significance: The identification of six GH9 enzymes from a compost metagenome and the functional variety of three characterized members highlight the importance of this enzyme family in bacterial biomass deconstruction.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-300006" status="VALID">
          <idno type="ROR">https://ror.org/03gcbhc33</idno>
          <orgName>IFP Energies nouvelles</orgName>
          <orgName type="acronym">IFPEN</orgName>
          <desc>
            <address>
              <addrLine>1-4 avenue de Bois Préau92500 Rueil-Malmaison</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.ifpenergiesnouvelles.fr/</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-1002356" status="VALID">
          <idno type="IdRef">149981651</idno>
          <idno type="RNSR">201220260Z</idno>
          <idno type="ROR">https://ror.org/04jm8zw14</idno>
          <orgName>Architecture et fonction des macromolécules biologiques</orgName>
          <orgName type="acronym">AFMB</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>Faculté des Sciences de Luminy - Case 932 163, Avenue de Luminy 13288 Marseille Cedex 09</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.afmb.univ-mrs.fr/</ref>
          </desc>
          <listRelation>
            <relation name="UMR7257" active="#struct-198056" type="direct"/>
            <relation name="UMR7257" active="#struct-441569" type="direct"/>
            <relation name="UMR1408" active="#struct-577435" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-422761" status="VALID">
          <orgName>King Abdulaziz University</orgName>
          <desc>
            <address>
              <addrLine>Djeddah 21589</addrLine>
              <country key="SA"/>
            </address>
            <ref type="url">http://www.kau.edu.sa/home_english.aspx</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-198056" status="VALID">
          <idno type="IdRef">15863621X</idno>
          <idno type="ISNI">0000 0001 2176 4817</idno>
          <idno type="ROR">https://ror.org/035xkbk20</idno>
          <orgName>Aix Marseille Université</orgName>
          <orgName type="acronym">AMU</orgName>
          <date type="start">2012-01-01</date>
          <desc>
            <address>
              <addrLine>Aix-Marseille UniversitéJardins du Pharo58 Boulevard Charles Livon13284 Marseille cedex 7</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-amu.fr/</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-479" status="VALID">
          <idno type="IdRef">150008651</idno>
          <idno type="RNSR">201220357E</idno>
          <idno type="ROR">057zme681</idno>
          <orgName>Laboratoire de chimie bactérienne</orgName>
          <orgName type="acronym">LCB</orgName>
          <date type="start">2012</date>
          <desc>
            <address>
              <addrLine>31 Chemin Joseph Aiguier 13402 MARSEILLE CEDEX 20</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://lcb.cnrs-mrs.fr/</ref>
          </desc>
          <listRelation>
            <relation name="UMR7283" active="#struct-198056" type="direct"/>
            <relation name="UMR7283" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-577435" status="VALID">
          <idno type="ROR">https://ror.org/003vg9w96</idno>
          <orgName>Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement</orgName>
          <orgName type="acronym">INRAE</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
      </listOrg>
      <listOrg type="projects">
        <org type="anrProject" xml:id="projanr-35044" status="VALID">
          <idno type="anr">ANR-12-BIME-0006</idno>
          <idno type="program">Bio-Matières et Energies</idno>
          <orgName>Biomines</orgName>
          <desc>Exploration de la Biodiversité Microbienne pour l'Identification de Nouvelles Enzymes et Souches CBP</desc>
          <date type="start">2012</date>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>