Observation of arenavirus nucleoprotein heptamer assembly
Résumé
Arenaviruses are enveloped viruses containing a segmented, negative and ambisense single‐stranded RNA genome wrapped with a nucleoprotein. The nucleoprotein is the most abundant viral protein in infected cells and plays a critical role in both replication/transcription and virion assembly. The nucleoprotein associates with RNA to form a ribonucleoprotein complex, and this implies self‐assembly while the exact structure of this polymer is not yet known. Here we report a measurement of the full length Mopeia virus NP by negative stain Transmission Electron Microscopy. We observed ribonucleoprotein complex particles with diameter 15 ± 1 nm as well as symmetric circular heptamers of the same diameter, consistent with previous observations.
Domaines
Sciences du Vivant [q-bio]| Origine | Fichiers éditeurs autorisés sur une archive ouverte |
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