Permanent diaphragmatic deficits and spontaneous respiratory plasticity in a mouse model of incomplete cervical spinal cord injury - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Respiratory Physiology & Neurobiology Année : 2021

Permanent diaphragmatic deficits and spontaneous respiratory plasticity in a mouse model of incomplete cervical spinal cord injury

Résumé

High spinal cord injuries (SCI) lead to permanent respiratory insufficiency, and the search for new therapeutics to restore this function is essential. To date, the most documented preclinical model for high SCI is the rat cervical C2 hemisection. However, molecular studies with this SCI model are limited due to the poor availability of genetically modified specimens. The aim of this work was to evaluate the pathophysiology of respiratory activity following a cervical C2 injury at different times post-injury in a C57BL/6 mouse model. No significant spontaneous recovery of diaphragmatic activity was observed up to 30 days post-injury in eupneic condition. However, during a respiratory challenge, i.e. mild asphyxia, a partial restoration of the injured diaphragm was observed at 7 days post-injury, corresponding to the crossed phrenic phenomenon. Interestingly, the diaphragmatic recording between 2 respiratory bursts on the injured side showed an amplitude increase between 1–7 days post-injury, reflecting a change in phrenic motoneuronal excitability. This increase in inter-burst excitability returned to pre-injured values when the crossed phrenic phenomenon started to be effective at 7 days post-injury. Taken together, these results demonstrate the ability of the mouse respiratory system to express long-lasting plasticity following a C2 cervical hemisection and genetically modified animals can be used to study the pathophysiological effects on these plasticity phenomena.
Fichier principal
Vignette du fichier
S1569904820302263.pdf (2.12 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03141460 , version 1 (07-11-2022)

Licence

Paternité - Pas d'utilisation commerciale

Identifiants

Citer

Pauline Michel-Flutot, Arnaud Mansart, Thérèse B. Deramaudt, Isley Jesus, Kun Ze Lee, et al.. Permanent diaphragmatic deficits and spontaneous respiratory plasticity in a mouse model of incomplete cervical spinal cord injury. Respiratory Physiology & Neurobiology, 2021, 284, ⟨10.1016/j.resp.2020.103568⟩. ⟨hal-03141460⟩
48 Consultations
10 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More