Single-nucleus RNA-seq and FISH identify coordinated transcriptional activity in mammalian myofibers - Archive ouverte HAL Access content directly
Journal Articles Nature Communications Year : 2020

Single-nucleus RNA-seq and FISH identify coordinated transcriptional activity in mammalian myofibers

Matthieu dos Santos
  • Function : Author
Stéphanie Backer
  • Function : Author
Benjamin Saintpierre
  • Function : Author
Brigitte Izac
Muriel Andrieu
  • Function : Author
Franck Letourneur
  • Function : Author
Athanassia Sotiropoulos
  • Function : Author
Pascal Maire
  • Function : Author
  • PersonId : 981575

Abstract

Skeletal muscle fibers are large syncytia but it is currently unknown whether gene expression is coordinately regulated in their numerous nuclei. Here we show by snRNA-seq and snATAC-seq that slow, fast, myotendinous and neuromuscular junction myonuclei each have different transcriptional programs, associated with distinct chromatin states and combinations of transcription factors. In adult mice, identified myofiber types predominantly express either a slow or one of the three fast isoforms of Myosin heavy chain (MYH) proteins, while a small number of hybrid fibers can express more than one MYH. By snRNA-seq and FISH, we show that the majority of myonuclei within a myofiber are synchronized, coordinately expressing only one fast Myh isoform with a preferential panel of muscle-specific genes. Importantly, this coordination of expression occurs early during post-natal development and depends on innervation. These findings highlight a previously undefined mechanism of coordination of gene expression in a syncytium.
Fichier principal
Vignette du fichier
s41467-020-18789-8.pdf (4.73 Mo) Télécharger le fichier
Origin Publisher files allowed on an open archive

Dates and versions

hal-03132559 , version 1 (05-02-2021)

Identifiers

Cite

Matthieu dos Santos, Stéphanie Backer, Benjamin Saintpierre, Brigitte Izac, Muriel Andrieu, et al.. Single-nucleus RNA-seq and FISH identify coordinated transcriptional activity in mammalian myofibers. Nature Communications, 2020, 11, ⟨10.1038/s41467-020-18789-8⟩. ⟨hal-03132559⟩

Collections

IMRB UPEC ANR
43 View
31 Download

Altmetric

Share

Gmail Mastodon Facebook X LinkedIn More