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Article Dans Une Revue Journal of Colloid and Interface Science Année : 2021

In vitro solubilization of fat-soluble vitamins in structurally defined mixed intestinal assemblies

Résumé

The structures of fed state intestinal assemblies containing bile components, dietary fat, and fat-solublevitamins are not well known, although they are involved in lipid transport. In this study, several methodswere used to investigate structural transitions upon various dietary lipids or various fat-soluble vitaminsincorporation in bile intestinal assemblies. In particular, DLS and turbidimetry were used to study tran-sition points as a function of component concentration, and cryo-TEM and SAXS were used to resolveassembly structures at microscopic and supramolecular scales, respectively. Results showed that increas-ing the concentration of dietary lipids in bile assembly induced a transition from core-shell micelles tounilamellar vesicles (except with caprylate lipids, always yielding micelles). In these specific assemblies,increasing the concentration of a fat-soluble vitamin either induced a systematic structural transition,defining a solubilization capacity (a-tocopherol or phylloquinone), or induced a structural transition onlyin micelles (retinol), or did not induce any structural transition up to very high concentrations (cholecal-ciferol). Using SAXS data, ideal molecular organizations are proposed for assemblies in the absence orpresence ofa-tocopherol.
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Dates et versions

hal-03122063 , version 1 (28-01-2021)

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Asma El Aoud, Emmanuelle Reboul, Aurélien Dupont, Cristelle Mériadec, Franck Artzner, et al.. In vitro solubilization of fat-soluble vitamins in structurally defined mixed intestinal assemblies. Journal of Colloid and Interface Science, 2021, 589, pp.229-241. ⟨10.1016/j.jcis.2021.01.002⟩. ⟨hal-03122063⟩
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