New Role for Serum Response Factor in Postnatal Skeletal Muscle Growth and Regeneration via the Interleukin 4 and Insulin-Like Growth Factor 1 Pathways - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular and Cellular Biology Année : 2006

New Role for Serum Response Factor in Postnatal Skeletal Muscle Growth and Regeneration via the Interleukin 4 and Insulin-Like Growth Factor 1 Pathways

Claude L. Charvet
Ara Parlakian
Julien Giordani
  • Fonction : Auteur
Anne Bertrand
Laure Renou
  • Fonction : Auteur
Alain Schmitt
  • Fonction : Auteur
Judith Melki
  • Fonction : Auteur
  • PersonId : 859966
Zhenlin Li
Dominique Daegelen
  • Fonction : Auteur correspondant
  • PersonId : 1089414

Connectez-vous pour contacter l'auteur
David Tuil
  • Fonction : Auteur correspondant
  • PersonId : 1089415

Connectez-vous pour contacter l'auteur

Résumé

Serum response factor (SRF) is a crucial transcriptional factor for muscle-specific gene expression. We investigated SRF function in adult skeletal muscles, using mice with a postmitotic myofiber-targeted disruption of the SRF gene. Mutant mice displayed severe skeletal muscle mass reductions due to a postnatal muscle growth defect resulting in highly hypotrophic adult myofibers. SRF-depleted myofibers also failed to regenerate following injury. Muscles lacking SRF had very low levels of muscle creatine kinase and skeletal alpha-actin (SKA) transcripts and displayed other alterations to the gene expression program, indicating an overall immaturity of mutant muscles. This loss of SKA expression, together with a decrease in beta-tropomyosin expression, contributed to myofiber growth defects, as suggested by the extensive sarcomere disorganization found in mutant muscles. However, we observed a downregulation of interleukin 4 (IL-4) and insulin-like growth factor 1 (IGF-1) expression in mutant myofibers which could also account for their defective growth and regeneration. Indeed, our demonstration of SRF binding to interleukin 4 and IGF-1 promoters in vivo suggests a new crucial role for SRF in pathways involved in muscle growth and regeneration.

Dates et versions

hal-03120739 , version 1 (25-01-2021)

Identifiants

Citer

Claude L. Charvet, Christophe Houbron, Ara Parlakian, Julien Giordani, Charlotte Lahoute, et al.. New Role for Serum Response Factor in Postnatal Skeletal Muscle Growth and Regeneration via the Interleukin 4 and Insulin-Like Growth Factor 1 Pathways: SKELETAL MUSCLE GROWTH, REGENERATION, AND SRF DEFECT. Molecular and Cellular Biology, 2006, 26 (17), pp.6664-6674. ⟨10.1128/MCB.00138-06⟩. ⟨hal-03120739⟩
27 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More