Uranium modifies or not behavior and antioxidant status in the hippocampus of rats exposed since birth
Résumé
In view of the known sensitivity of the developing central nervous system to pollutants, we sought to assess the effects of exposure to uranium (U) — a heavy metal naturally present in the environment — on the behavior of young rats and the impact of oxidative stress on their hippocampus. Pups drank U (in the form of uranyl nitrate) at doses of 10 or 40 mg.L-1 for 10 weeks from birth. Control rats drank mineral water. Locomotor activity in an open field and practice effects on a rotarod device decreased in rats exposed to 10 mg.L-1 (respectively, -19.4% and -51.4%) or 40 mg.L-1 (respectively, -19.3% and -55.9%) in compared with control rats. Anxiety (+37%) and depressive-like behavior (-50.8%) were altered by U exposure only at 40 mg.L-1. Lipid peroxidation (+35%) and protein carbonyl concentration (+137%) increased significantly after exposure to U at 40 mg.L-1. A significant increase in superoxide dismutase (SOD, +122.5%) and glutathione peroxidase (GPx, +13.6%) activities was also observed in the hippocampus of rats exposed to 40 mg.L-1. These results demonstrate that exposure to U since birth alters some behaviors and modifies antioxidant status. © 2015, Japanese Society of Toxicology. All rights reserved.
Mots clés
catalase
drinking water
glutathione peroxidase
superoxide dismutase
uranium
pollutant
animal experiment
animal tissue
anxiety
Article
body weight
colorimetry
controlled study
depression
elevated plus maze test
environmental exposure
enzyme activity
experimental behavioral test
female
food intake
forced swim test
hippocampus
lipid peroxidation
locomotion
male
mass spectrometry
nonhuman
open field test
oxidative stress
rat
rotarod test
spectrophotometry
adverse effects
animal
animal behavior
chemically induced
dose response
drug effects
metabolism
motor activity
pregnancy
protein carbonylation
sexual development
Sprague Dawley rat
toxicity
Rattus
Animals
Anxiety
Behavior
Animal
Depression
Dose-Response Relationship
Drug
Environmental Exposure
Environmental Pollutants
Female
Glutathione Peroxidase
Hippocampus
Lipid Peroxidation
Male
Motor Activity
Oxidative Stress
Pregnancy
Protein Carbonylation
Rats
Sprague-Dawley
Sex Characteristics
Superoxide Dismutase
Uranium