Bone marrow stromal cells spontaneously produce Flt3-ligand: Influence of ionizing radiations and cytokine stimulation
Résumé
Purpose: To define the ability of human bone marrow (BM) stromal cells to produce fms-like tyrosine kinase 3 (Flt3)-ligand (FL), and the effect of irradiation, tumour necrosis factor-alpha (TNF[image omitted]) or tumour growth factor beta (TGF[image omitted]) on FL production. Material and methods: Primary BM stromal cell cultures were irradiated at 2-10 Gy or were stimulated with TNFα or TGFβ1. The presence of FL was tested in culture supernatants and in cell lysate. The presence of a membrane-bound form of FL and the level of gene expression were also tested. Results: Primary BM stromal cells spontaneously released FL. This production was increased by TNFα but not by TGFβ1 or by irradiation. Chemical induction of osteoblastic differentiation from BM stromal cells also induced an increase in FL release. Conclusions: Our results suggest that the observed increase in FL concentration after in vivo irradiation is an indirect effect. The possible implication of BM stromal cells in these mechanisms is discussed. © 2008 Informa UK Ltd.
Mots clés
Flt3 ligand
transforming growth factor beta
tumor necrosis factor alpha
article
bone marrow cell
cell culture
cell differentiation
cell lysate
cell stimulation
clinical article
controlled study
drug effect
enzyme activation
enzyme linked immunosorbent assay
enzyme synthesis
gene expression
human
human cell
human tissue
ionizing radiation
phenotype
priority journal
stroma cell
supernatant
Bone Marrow Cells
Cell Differentiation
Cells
Cultured
Humans
Membrane Proteins
Osteoblasts
Phenotype
Stromal Cells
Transforming Growth Factor beta1
Tumor Necrosis Factor-alpha