Mass spectrometry imaging (MSI) using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS) has been employed for the elemental bio-distribution and quantification of uranium (U) in histological tissue sections of rodent kidneys. Samples were immediately immersed into 4% paraformaldehyde (PFA) solution during 24h, Tissue Tek OCT embedded and stored at -80°C until cutting in cryostat and mounting in gel covered glass slides. Sample preparation and laser conditions were carefully optimized to assure the complete ablation of the sample matrix. This works presents the development of internal standard correction methodology based on doped gelatine. In order to correct matrix effects, lack of tissue homogeneity and instrumental drift, a thulium (Tm, internal standard) spiked 10% (m/v) gel was prepared and deposited on the top of glass slides. For quantification purposes, matrix-matched laboratory standards were prepared from a pool of rat kidneys by dosing each level with different concentrations of uranium. The quantitative images of cryo-sections revealed heterogeneous distribution of uranium within the renal tissue, being the cortical concentration up to 120-fold higher than the medullary concentration.