Hybrid composites films were prepared by the incorporation of titanium dioxide doped silver nanoparticles (TiO 2-Ag NPs) at different concentrations (1, 2, 3 and 4 wt%) into Rhinobatos cemiculus gelatin (RCG) matrix. The microstructure analysis, realized by scanning electron microscopy and atomic force microscopy revealed a homogenous structure and a smooth surface of the composite films. The mechanical properties, light transmission and moisture content of composite films are greatly dependent on the amount of added TiO 2-Ag NPs. A gradual decrease in elongation at break was observed with the increase in TiO 2-Ag nanoparticles content (reaching 16.05% at an addition level of 4%). Moreover, the addition of TiO 2-Ag NPs considerably enhanced the antioxidant activity of nanocomposite films evaluated by DPPH radical scavenging activity and ferrous chelating activity. It is, thus, concluded that the incorporation of TiO 2-Ag NPs into the gelatin matrix promotes the improvement of gelatin films' performance.