Amplified by the development of new technologies, the interest in personal performance has been growing over the last years. Acceleration has proved to be an easy variable to collect, and was addressed in several works. However, few of them evaluate the effect of running speed on relevant indicators. The influence of the sensors location on the measurement is rarely studied as well. This study is dedicated to investigating the effect of running speed on acceleration measured at three different positions on 18 volunteers. All participants were equipped with three inertial measurement units: on the dorsal surface of the right foot (Fo), at the centre of gravity of the tibia (Ti), at the L4-L5 lumbar (Lu). The test was performed on a treadmill at nine randomised speeds between 8 and 18 km/h. Ten accelerometric variables were calculated. Linear regressions were used to calculate speed from the indicators calculated on (Lu), (Ti), (Fo). Indicators associated to signal energy were highly correlated with speed (r2>0.90). Median frequency appears to be affected by the frequency resolution. Finally, the measurement points closest to the impact zone result in the most correlated indicators.