The extremely diverse service requirements is an important challenge for the future generations of wireless communication technologies. As promising solutions, orthogonal frequency division multiplexing (OFDM)-based massive multiple-input multiple-output (MIMO) and mixed numerologies transmission are proposed. This letter investigates the transmission of different users, sharing the same bandwidth while they are associated to different numerologies. A transceiver design which aims to manage the mixed numerology spectrum sharing (SS) transmission is first presented. Then, we analyze the inter-numerology interference (INI) and derive its corresponding theoretical expressions. Based on the derived closed-form expressions, a new INI cancellation scheme is introduced, which efficiently mitigates the INI and enhances the performance of massive MIMO-OFDM uplink systems.