Determination of strain path across martensitic transformation in materials with two transformation orientation relationships (ORs) is challenging due to the temporal and spatial limitation of the modern techniques. In this work, an analyzing strategy, i.e., the determination of transformation path via the variant organization feature of martensite, was suggested and applied for the Ni-Mn-based alloys as an example, based on the consideration that the different crystallographic symmetries of transformation systems will result in distinct organizations of martensite variants. For the selected Ni-Mn-based alloys, the orientation examination revealed that both the K−S and the Pitsch OR are respected. Further analyses in terms of the strain and stress compatibility condition and the minimum energy criteria showed that, theoretically, the K−S path produces 2 variants as a self-accommodated variant group, whereas the Pitsch path produces 4 variants as a self-accommodated variant group. Compared with the experimental results, the Pitsch path is the energetically favorable transformation path and actually occurs in stress-free austenite of the Ni−Mn based alloys. The significance of this work is multi-fold. It first resolves the long puzzling issue of transformation strain path of Ni-Mn-based alloys. Moreover, the analyzing scheme can be generalized to determine the transformation characters for martensitic transformation in other systems with multiple possible transformation orientation relationships.