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Communication Dans Un Congrès Année : 2020

Sensitive and specific detection of Ewing sarcoma minimal residual disease in ovarian and testicular tissues

Victoria Grèze
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Halle Pascale
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Robin Nina
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Véronèse Lauren
Lejeune Hervé
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Durand Philippe
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Martin Guillaume
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Sandra Sanfilippo
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Canis Michel
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Kanold Justyna
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Tchirkov Andrei
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Résumé

Sensitive and specific detection of Ewing sarcoma minimal residual disease in ovarian and testicular tissues Ewing sarcoma (EWS) is a common pediatric solid tumor with high metastatic potential. Due to toxic effects of treatments on reproductive functions, the cryopreservation of ovarian tissue (OT) or testicular tissue (TT) is recommended to preserve fertility. However, the risk to reintroduce residual metastatic tumor cells should be evaluated before fertility restoration. Our goal was to validate a sensitive and specific approach for EWS minimal residual disease (MRD) detection in frozen germinal tissues. Thawed OT (n=12) and TT (n=14) were contaminated with tumor RD-ES cells (10, 100 and 1000 cells) and EWS-FLI1 tumor-specific transcript was quantified with RT-qPCR. All contaminated samples were found to be positive, with the strong correlation between RD-ES cell numbers and EWS-FLI1 levels in OT (r=0.93) and TT (r=0.96) (P<0.001). No transcript was detected in uncontaminated control samples. The invasive potential of Ewing cells was evaluated using co-culture techniques. After co-culturing, tumor cells were detected in OT/TT with histology, FISH and RT-qPCR. In addition, 4 OT and 4 TT samples from children with metastatic EWS were tested, and no MRD was found using RT-qPCR and histology. We demonstrated the high sensitivity and specificity of RT-qPCR to detect EWS MRD in OT/TT samples.
BLEFCO Sensitive and Specific Detection of Ewing Sarcoma Minimal Residual Disease in Ovarian and Testicular Tissues in an In Vitro Model.pdf (359.11 Ko) Télécharger le fichier
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Dates et versions

hal-03066130 , version 1 (15-12-2020)

Identifiants

  • HAL Id : hal-03066130 , version 1

Citer

Chaput Laure, Victoria Grèze, Halle Pascale, Robin Nina, Pereira Bruno, et al.. Sensitive and specific detection of Ewing sarcoma minimal residual disease in ovarian and testicular tissues. BLEFCO CECOS, 2020, Paris, France. ⟨hal-03066130⟩
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