Unique genome replication mechanism of the archaeal virus AFV1 - Archive ouverte HAL
Article Dans Une Revue Molecular Microbiology Année : 2014

Unique genome replication mechanism of the archaeal virus AFV1

Résumé

The exceptional genomic content and genome organization of the Acidianus filamentous virus 1 (AFV1) that infects the hyperthermophilic archaeon Acidianus hospitalis suggest that this virus might exploit an unusual mechanism of genome replication. An analysis of replicative intermediates of the viral genome by two-dimensional (2D) agarose gel electrophoresis revealed that viral genome replication starts by the formation of a D-loop and proceeds via strand displacement replication. Characterization of replicative intermediates using dark-field electron microscopy, in combination with the 2D agarose gel electrophoresis data, suggests that recombination plays a key role in the termination of AFV1 genome replication through the formation of terminal loops. A terminal protein was found to be attached to the ends of the viral genome. The results allow us to postulate a model of genome replication that relies on recombination events for initiation and termination.
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hal-03065162 , version 1 (14-12-2020)

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Mery Pina, Tamara Basta, Tessa Quax, Alexandra Joubert, Sonia Baconnais, et al.. Unique genome replication mechanism of the archaeal virus AFV1. Molecular Microbiology, 2014, 92 (6), pp.1313-1325. ⟨10.1111/mmi.12630⟩. ⟨hal-03065162⟩
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