Activation of TRPA1 Channel by Antibacterial Agent Triclosan Induces VEGF Secretion in Human Prostate Cancer Stromal Cells - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cancer Prevention Research Année : 2017

Activation of TRPA1 Channel by Antibacterial Agent Triclosan Induces VEGF Secretion in Human Prostate Cancer Stromal Cells

Sandra Derouiche
  • Fonction : Auteur
Pascal Mariot
Marine Warnier
  • Fonction : Auteur
Eric Vancauwenberghe
  • Fonction : Auteur
Pierre Gosset
Brigitte Mauroy
  • Fonction : Auteur
Jean-Louis Bonnal
  • Fonction : Auteur
Christian Slomianny
  • Fonction : Auteur
Philippe Delcourt
  • Fonction : Auteur
Etienne Dewailly
  • Fonction : Auteur
Natalia Prevarskaya
Morad Roudbaraki
  • Fonction : Auteur
  • PersonId : 937217

Résumé

Accruing evidence indicates that exposure to environmental compounds may adversely affect human health and promote carcinogenesis. Triclosan (TCS), an antimicrobial agent widely used as a preservative in personal care products, has been shown to act as an endocrine disruptor in hormone-dependent tissues. Here, we demonstrate a new molecular mechanism by which TCS stimulates the secretion by human prostate cancer stromal cells of vascular endothelial growth factor (VEGF), a factor known to promote tumor growth. This mechanism involves an increase in intracellular calcium levels due to the direct activation of a membrane ion channel. Using calcium imaging and electrophysiology techniques, we show for the first time that environmentally relevant concentrations of TCS activate a cation channel of the TRP family, TRPA1 (Transient Receptor Potential Ankirin 1), in primary cultured human prostate cancer stromal cells. The TCS-induced TRPA1 activation increased basal calcium in stromal cells and stimulated the secretion of VEGF and epithelial cells proliferation. Interestingly, immunofluorescence labeling performed on formalin-fixed paraffin-embedded prostate tissues showed an exclusive expression of the TRPA1 channel in prostate cancer stromal cells. Our data demonstrate an impact of the environmental factor TCS on the tumor microenvironment interactions, by activating a tumor stroma-specific TRPA1 ion channel.
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Dates et versions

hal-03060361 , version 1 (13-12-2020)

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Sandra Derouiche, Pascal Mariot, Marine Warnier, Eric Vancauwenberghe, Gabriel Bidaux, et al.. Activation of TRPA1 Channel by Antibacterial Agent Triclosan Induces VEGF Secretion in Human Prostate Cancer Stromal Cells. Cancer Prevention Research, 2017, 10 (3), pp.177-187. ⟨10.1158/1940-6207.CAPR-16-0257⟩. ⟨hal-03060361⟩
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