Cold/menthol TRPM8 receptors initiate the cold‐shock response and protect germ cells from cold‐shock–induced oxidation - Archive ouverte HAL
Article Dans Une Revue FASEB Journal Année : 2016

Cold/menthol TRPM8 receptors initiate the cold‐shock response and protect germ cells from cold‐shock–induced oxidation

Anne‐sophie Borowiec
  • Fonction : Auteur
Benoit Sion
Fréric Chalmel
  • Fonction : Auteur
Antoine Rolland
Loïc Lemonnier
Tatiana de Clerck
  • Fonction : Auteur
Alexandre Bokhobza
  • Fonction : Auteur
Sandra Derouiche
  • Fonction : Auteur
Etienne Dewailly
  • Fonction : Auteur
Christian Slomianny
  • Fonction : Auteur
Claire Mauduit
  • Fonction : Auteur
Mohamed Benahmed
  • Fonction : Auteur
Morad Roudbaraki
  • Fonction : Auteur
Bernard Jégou
Natalia Prevarskaya

Résumé

Testes of most male mammals present the particularity of being externalized from the body and are consequently slightly cooler than core body temperature (4-8°C below). Although, hypothermia of the testis is known to increase germ cells apoptosis, little is known about the underlying molecular mechanisms, including cold sensors, transduction pathways, and apoptosis triggers. In this study, using a functional knockout mouse model of the cold and menthol receptors, dubbed transient receptor potential melastatine 8 (TRPM8) channels, we found that TRPM8 initiated the cold-shock response by differentially modulating cold-and heat-shock proteins. Besides, apoptosis of germ cells increased in proportion to the cooling level in control mice but was independent of temperature in knockout mice. We also observed that the rate of germ cell death correlated positively with the reactive oxygen species level and negatively with the expression of the detoxifying enzymes. This result suggests that the TRPM8 sensor is a key determinant of germ cell fate under hypothermic stimulation.
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hal-03060358 , version 1 (13-12-2020)

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Anne‐sophie Borowiec, Benoit Sion, Fréric Chalmel, Antoine Rolland, Loïc Lemonnier, et al.. Cold/menthol TRPM8 receptors initiate the cold‐shock response and protect germ cells from cold‐shock–induced oxidation. FASEB Journal, 2016, 30 (9), pp.3155-3170. ⟨10.1096/fj.201600257R⟩. ⟨hal-03060358⟩
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