PERIOD-controlled deadenylation of the timeless transcript in the Drosophila circadian clock - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Proceedings of the National Academy of Sciences of the United States of America Année : 2019

PERIOD-controlled deadenylation of the timeless transcript in the Drosophila circadian clock

Résumé

The Drosophila circadian oscillator relies on a negative transcriptional feedback loop, in which the PERIOD (PER) and TIMELESS (TIM) proteins repress the expression of their own gene by inhibiting the activity of the CLOCK (CLK) and CYCLE (CYC) transcription factors. A series of posttranslational modifications contribute to the oscillations of the PER and TIM proteins but few posttranscriptional mechanisms have been described that affect mRNA stability. Here we report that down-regulation of the POP2 deadenylase, a key component of the CCR4-NOT deadenylation complex, alters behavioral rhythms. Down-regulating POP2 specifically increases TIM protein and tim mRNA but not tim pre-mRNA, supporting a posttranscriptional role. Indeed, reduced POP2 levels induce a lengthening of tim mRNA poly(A) tail. Surprisingly, such effects are lost in per 0 mutants, supporting a PER-dependent inhibition of tim mRNA deadenylation by POP2. We report a deadenylation mechanism that controls the oscillations of a core clock gene transcript.

Dates et versions

hal-03055138 , version 1 (11-12-2020)

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Brigitte Grima, Christian Papin, Béatrice Martin, Elisabeth Chélot, Prishila Ponien, et al.. PERIOD-controlled deadenylation of the timeless transcript in the Drosophila circadian clock. Proceedings of the National Academy of Sciences of the United States of America, 2019, 116 (12), pp.5721-5726. ⟨10.1073/pnas.1814418116⟩. ⟨hal-03055138⟩
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