Dynamic single-cell phenotyping of immune cells using the microfluidic platform DropMap
Résumé
This protocol describes a microfluidic platform for dynamic high-throughput analysis the phenotype of single cells. Cell-surface markers and secreted proteins are quantified and characterized by fluorescence detection using tailored immunoassays, simultaneously with measurement of other cellular characteristics, including endocytosis activity and viability. TWEET A new protocol describes a microfluidics-based assay for high-throughput interrogation of protein secretion kinetics in single cells. @BIOASTER @EyerFira @ETH_DCHAB COVER TEASER Microfluidics-based analysis of protein secretion Up to three primary research articles where the protocol has been used and/or developed.
Domaines
Matière Molle [cond-mat.soft]| Origine | Fichiers produits par l'(les) auteur(s) |
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