Amphiphilic block copolymers poly(ethylene glycol)-block-poly(N-3-(methylthio)propyl glycine) (PEGb-PMeSPG) were synthesized via ring-opening polymerization of N-3-(methylthio)propyl glycine Nthiocarboxyanhydride (MeSPG-NTA) initiated by amino-terminated PEG. The self-assemblies of three PEG-b-PMeSPG copolymers with different PMeSPG block lengths were first prepared by nanoprecipitation method using THF and DMF, respectively, as the organic solvent, and their morphologies were studied by Cryo-EM and DLS. To prepare polymersomes loaded with glucose oxidase (GOx), double emulsion method followed by extrusion treatment was employed. The oxidation-responsive disruption of polymersomes was achieved upon the introduction of glucose because of the oxidants generated insitu by GOx/glucose.